γ-L-Glutamyl-3-carboxy-4-nitroanilide, ityuwa ye-monoammonium Cas: 63699-78-5 Ukukhanya okutyheli kwe-microcrystalline powder
| Inombolo Yekhathalogu | XD90183 |
| Igama lemveliso | γ-L-Glutamyl-3-carboxy-4-nitroanilide, ityuwa ye-monoammonium |
| CAS | 63699-78-5 |
| Ifomula yeemolekyuli | C12H12N3O7-.H4N+ |
| Ubunzima beMolekyuli | 328.27 |
| Iinkcukacha zokuGcina | I-Ambient |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo we-microcrystalline otyheli |
| uAsay | 99% |
| Indawo yokunyibilika | 187ºC ± 2.0ºC |
| Indawo yokubilisa | 718.3 ° C kwi-760 mmHg |
| Indawo yokuflasha | 368.5 °C |
| Ukunyibilika | I-H2O: 100 mg / mL, ecacileyo, ephuzi-luhlaza |
I-kinetics ye-serum ye-gamma-glutamyltransferase (EC 2.3.2.2) yaphandwa, ngokusetyenziswa kwe-glycylglycine njenge-gamma-glutamyl yokwamkela i-substrate kunye ne-gamma-glutamyl-4-nitroanilide kunye ne-carboxy derivative, i-gamma-glutamyl-3-carboxy-4 -nitroanilide, njengama-substrates abanikeli.Ukwenzeka ngaxeshanye kuzo zombini i-gamma-glutamyltransfer kunye ne-autotransfer yasekwa ngokuhla kwechromatography yephepha.Ulungelelwaniso oluqhubekayo lwe-double-reciprocal plots luqinisekisa ukuba indlela ye-enzyme ayinanto (ping-pong bi-bi).Ukuthintelwa nguwuphi na umnikezeli akuzange kufunyanwe, kwaye ukuvinjwa kwe-glycylglycine kwabonwa kuphela kugxininiso olungaphezulu kwezo zomdla weklinikhi.I-Kinetic constants efunyenwe ngokuhlalutya okungahambi kakuhle kwedatha yesantya sokuqala yayisetyenziselwa ukumisela i-reagent substrate concentrations yovavanyo lwale enzyme.Uvavanyo olusebenzisa i-4 mmol ye-gamma-glutamyl-3-carboxy-4-nitroanilide kunye ne-100 mmol ye-glycylglycine ilitha nganye luvelise imisebenzi elinganayo naleyo ngokuvavanywa kusetyenziswa i-4 mmol ye-gamma-glutamyl-4-nitroanilide kunye ne-40 mmol glycylglycine ilitha nganye.Oku kugxininiswa komxhasi we-carboxy kunye ne-glycylglycine "zixabisa kakhulu" kwaye azikho zingxaki zenkqubo xa zisetyenziswa.
