Ibhaktiriya ye-entomopathogenic, i-Xenorhabdus nematophila, yenza ukuba i-immunosuppression yezinambuzane ezijoliswe kuyo inqande umsebenzi we-phospholipase A (2) (PLA (2)).Kutshanje, i-PLA (2) enxulumene nofuzo ichongiwe kwibhungane lomgubo obomvu, i-Tribolium castaneum.Olu phononongo ludibanise le PLA (2) gene kwi-bacterial expression vector ukuvelisa i-enzayimi ephinda iphinde yakhiwe.I-recombinant T. castaneum PLA (2) (TcPLA (2)) ibonise umsebenzi wayo we-enzyme ene-substrate concentration, i-pH, kunye nobushushu be-ambient.Iimpawu zayo ze-biochemical zihambelana nohlobo oluyimfihlo lwe-PLA (2) (sPLA (2)) ngenxa yokuba umsebenzi wayo wawuvinjelwe yi-dithiothreitol (i-agent yokunciphisa i-disulfide bond) kunye ne-bromophenacyl bromide (i-sPLA ethile (2) inhibitor) kodwa kungekhona nge-methylarachidonyl. i-fluorophosphonate (uhlobo oluthile lwe-cytosolic ye-PLA (2)).I-X. nematophila culture broth iqulethe i-PLA (2) inhibitory factor (s), eyayininzi kakhulu kwimidiya efunyenwe kwisigaba sokukhula kwebhaktheriya emileyo.I-PLA (2) inhibitory factor (s) yayinganyangeki ukushisa kwaye ikhutshwe kuwo omabini amaqhezu amanzi kunye ne-organic.Impembelelo ye-PLA (2)-inhibitory fraction kwi-immunosuppression ye-T. castaneum yayifaniswa ngokulinganayo kunye nesiphumo sokuvinjelwa kwe-TcPLA (2) ye-gene expression ngokuphazamiseka kwe-RNA.
