I-2'-Deoxythymidine Cas: 50-89-5
| Inombolo Yekhathalogu | XD90582 |
| Igama lemveliso | 2'-Deoxythymidine |
| CAS | 50-89-5 |
| Ifomula yeemolekyuli | I-C10H14N2O5 |
| Ubunzima beMolekyuli | 242.23 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe ukuya kumhlophe |
| Isivavanyi | 99% |
| Ilahleko ekomisweni | <0.5% |
| Intsalela kwi-Ignition | <0.3% |
| Ukujikeleza okuthe ngqo | +17.5 - +19.5 |
Ngaphambili siye sadibana neenkqubo zokulawula apho i-dihydrotestosterone (DHT) isebenzise impembelelo yayo yokuthintela kwiprotheni enxulumene nehomoni ye-parathyroid (PTHrP) yoxinzelelo lofuzo ngokusebenzisa i-estrogen receptor (ER) α, kodwa kungekhona i-androgen receptor (AR), kumhlaza wesifuba i-MCF-7 iiseli. .Apha, siye saphanda ukuba ngaba i-aberrant ligand-nuclear receptor (NR) ikhona na kwi-prostate cancer LNCaP cells.Okokuqala, siqinisekisile ukuba iiseli ze-LNCaP zibonise ubuninzi be-AR kumanqanaba angenanto ye-ERα / β okanye i-progesterone receptor.Bobabini ukunyanzeliswa kwe-PTHrP kunye nokusebenza kwe-prostate-specific antigen genes kwabonwa emva kokulawulwa ngokuzimeleyo kwe-17β-estradiol (E2), i-DHT, okanye i-R5020.Ngokungqinelana noluvo lokuba i-LNCaP AR iphulukene nokuchaneka kwe-ligand ngenxa yotshintsho (Thr-Ala877), imifuniselo ye-siRNA ejolise kwi-NR echaphazelekayo ibonise ukuba i-AR yalawula indima yomlamli wolawulo oluxhomekeke kwihomoni ekwabelwanayo ngayo, eyayinxulunyaniswa ngokungaguquguqukiyo. ngenyukliya translocatio n yale AR eguqukayo.Uhlalutyo lwe-Microarray yokulawulwa kofuzo yi-DHT, i-E2, okanye i-R5020 idize ukuba ngaphezu kwesiqingatha sezakhi zofuzo ezantsi kwe-AR (Thr-Ala877) zidibene kwiiseli ze-LNCaP.Umdla okhethekileyo, saqonda ukuba i-AR (uhlobo lwasendle [wt]) kunye ne-AR (Thr-Ala877) yayinoxanduva ngokulinganayo kwintsebenziswano ye-E2-AR.Iimvavanyo ze-microscopy ze-Fluorescence zibonise ukuba zombini i-EGFP-AR (wt) kunye ne-EGFP-AR (Thr-Ala877) zaye zabekwa kuphela ngaphakathi kwi-nucleus emva kwe-E2 okanye unyango lwe-DHT.Ngaphaya koko, iimvavanyo zentatheli zibonise ukuba ezinye iiseli zomhlaza zibonise i-E2-AR (wt) engaqhelekanga ebonisa okufanayo kwiiseli ze-LNCaP.Apha sichaza ubukho bonxibelelwano olubambeneyo phakathi kwe-wt AR kunye ne-E2 kwiiseli ezithile zomhlaza ezibuthathaka kwihomoni.
