I-2-Oxobutyric acid CAS: 600-18-0 intlama engenambala
| Inombolo Yekhathalogu | XD90263 |
| Igama lemveliso | 2-Oxobutyric acid |
| CAS | 600-18-0 |
| Ifomula yeemolekyuli | C4H6O3 |
| Ubunzima beMolekyuli | 102.089 |
| Iinkcukacha zokuGcina | -20 °C |
| IKhowudi yoMrhumo eHarmonized | 2918300090 |
Ukucaciswa kweMveliso
| Ukuxinana | 1.182 |
| Indawo yokunyibilika | 30-34 °C |
| Indawo yokubilisa | 84 °C |
| Imbonakalo | intlama engenambala |
| Isivavanyi | 99% |
I-1.Optically active D-2-hydroxybutanoate yibhloko ebalulekileyo yokwakha ephakathi kumayeza kunye ne-biodegradable poly(2-hydroxybutanoate).Isisombululo seKinetic ye-racemic 2-hydroxybutanoate inokuba yinto eluhlaza kunye nenqwenelekayo kwimveliso ye-D-2-hydroxybutanoate.Kulo msebenzi, i-D-2-hydroxybutanoate kwi-concentration ephezulu (0.197 M) kunye nokugqithisa kwe-enantiomeric (99.1%) yaveliswa yi-NAD-ezimeleyo ye-L-lactate dehydrogenase (L-iLDH) equkethe i-biocatalyst.I-2-Oxobutanoate, enye i-intermediate ebalulekileyo, yenziwe ngokubambisana kwi-concentration ephezulu (0.193 M).Ukusebenzisa inkqubo yokutshintshiselana nge-ion elula kunye ne-macroporous anion exchange resin D301, i-D-2-hydroxybutanoate yahlukana nenkqubo ye-biotransformation kunye nokubuyisela okuphezulu kwe-84.7%.
I-2.I-asymmetric synthesis ye-amino acid engekho ngokwemvelo yaboniswa yi-omega-transaminase esuka kwi-Vibrio fluvialis JS17.I-L-2-Aminobutyric acid yenziwe i-2-oxobutyric acid kunye ne-benzylamine ene-enantiomeric engaphezulu kwe-99%.Ukusabela kubonise ukuvinjelwa kwemveliso enzima ngu-benzaldehyde, eyaye yanqotshwa ngokusebenzisa inkqubo ye-biphasic reaction system ukususa imveliso yokuthintela kwisigaba samanzi.Kwimpendulo eqhelekileyo ye-biphasic (50 mM 2-oxobutyric acid, 70 mM benzylamine kunye ne-2.64 U / ml i-enzyme ecocekileyo) usebenzisa i-hexane njenge-extractant, ukuguqulwa kwe-2-oxobutyric acid kwafikelela kwi-96% kwi-5 h ngelixa kuphela i-39% yokuguqulwa ifunyenwe ngaphandle. ukukhutshwa kwemveliso.
