Iimveliso

Ukugxilwa kwe-submillimolar ye-cytotoxic bile acids (BAs) kubangela ukufa kweeseli nge-apoptosis.Ngakolunye uhlangothi, ii-BAs ezininzi ze-cytoprotective zaboniswa ukukhusela i-apoptosis kuluhlu olufanayo loxinaniso.Nangona kunjalo, iindlela ezithi ngazo ii-BAs ziqalise ezi ziphumo zomqondiso zahlukileyo zihlala zingacacanga.Olu pho nonongo lujolise ekuqinisekiseni ukuba i-cytotoxic kunye ne-cytoprotective BAs, kwi-physiologically active concentrations, ziyakwazi ukumodareyitha iipropathi ze-biophysical ze-lipid membranes, ezinokuthi ziguqulele kwiinguqu kwi-apoptotic threshold yeeseli.Ukubophelela kwee-BAs kwiinwebu kwavavanywa ngokwahluka kweeparamitha ze-fluorescence zee-BA ezifanelekileyo eziphuma kwi-dervatized.Ezi zinto ziphumayo zahlulahlulwe ngobudlelwane obuphezulu kulwelo oluphazamisekileyo kunolwelo olutyetyisiweyo lwe-cholesterol.Ii-BA ezingabhalwanga nazo zibonakaliswe ukuba zinendawo engaphezulu ekusebenzisaneni ne-lipid membrane.Ukongeza, ukusebenzisana kwee-cytotoxic BAs kunye ne-membrane kubangele ukwanda kwe-membrane, njengoko kubandakanyiwe kwidatha ye-FRET.Ngaphezu koko, kwaboniswa ukuba ii-BAs ze-cytotoxic ziye zakwazi ukuphazamisa ngokubonakalayo ukucwangciswa kwe-membrane nge-cholesterol ekugxilweni kwe-physiologically esebenzayo ye-BA, umphumo ongahambelani nokususwa kwe-cholesterol.Ngakolunye uhlangothi, i-cytoprotective bile acids yayingenayo impembelelo kwiipropati ze-membrane.Kwagqitywa ukuba, xa kujongwa iziphumo zokungagungqi kwe-membrane, umsebenzi we-apoptotic we-cytotoxic BAs unokunxulunyaniswa notshintsho kwi-plasma membrane organisation (umz. ukumodareyithwa kweendawo ze-lipid) okanye ngokunyuka kwe-mitochondrial membrane affinity ye-apoptotic proteins.