I-4-Nitrophenyl-alpha-D-galactopyranoside CAS: 7493-95-0 99%
| Inombolo Yekhathalogu | XD900018 |
| Igama lemveliso | I-4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Ifomula yeemolekyuli | C12H15NO8 |
| Ubunzima beMolekyuli | 301.25 |
| Iinkcukacha zokuGcina | -15 ukuya -20 °C |
| IKhowudi yoMrhumo eHarmonized | 29400000 |
Ukucaciswa kweMveliso
| Imbonakalo | Mhlophe ukuya kumgubo oLuhlaza ukuya kwikristale |
| Isivavanyi | 99% |
I-4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) i-substrate eyenziwe ye-4-nitrophenyl (pNP) glycopyranoside yokufumanisa umsebenzi we-α-galactosidase.Isixa se-pNP esikhutshwe sanda kakhulu xa i-4-Nitrophenyl α-D-galactopyranoside isetyenziswe njenge-substrate.
Usebenzisa4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPisuka kwi4-Nitrophenylα-D-galactopyranosideiphezulu kakhulu kuthatoftheotherpNP-glycopyranosidesatpH4.0apho ingekhocalpnylα-D-galactopyranosides iphezulu kakhulu kunetheotherpNP-glycopyranosidesatpH4.0apho ingekhocalpc-yabonwa-besbesdetected-besdetected. I-nyl (pNP) glycopyranosidesasartificialsubstrates ibonisathatyieldinliberatedgalactoseresiduesfrom4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, iethepHlevelwhereyieldthresholdisdeepestestestest.
I-Substrate ye-α-D-galactosidase (α-D-Galactosidase).Ibophelela kwi-E. coli lactose protein carrier.Phakathi kwe-α-D-galactose.
Indlela ekhawulezayo yokuvavanya umsebenzi we-lytic we-antimicrobial agents ngokumelene nemvubelo kunye nefungi iye yaphuhliswa.Uvavanyo lusekelwe ekukhululweni kwe-enzyme ye-intracellular, i-maltase (alpha-glucosidase).Umsebenzi okhutshiweyo we-maltase ulinganiswe nge-colorimetrically ngokuveliswa kwe-p-nitrophenol ukusuka kwi-p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Umsebenzi we-lytic we-antimicrobial compounds ezahlukeneyo walinganiswa ngokuchasene neeseli zegwele okanye i-spores ekhulayo ye-filamentous fungi.Umsebenzi we-Lytic wokuchasana negwele unokubonwa kwimizuzu engama-20 yokufukamela kwi-30 degrees C ngokuchasene ne-Saccharomyces cerevisiae, i-Candida albicans, kunye ne-Cryptococcus neoformans.Umsebenzi weLytic wokuchasana nokungunda ubonakale emva kweyure ezi-2 zokufukamela kuma-30 degrees C ngokuchasene neenkozo ezintshulayo ze-Aspergillus niger kunye ne-Botrytis cinerea.Iiseli ezipheleleyo zegwele okanye umngundo azizange zivelise i-PNPG eyaneleyo kwi-hydrolyze ngaphakathi kwe-3 h kuma-30 degrees C ukuze zivelise utshintsho olubonakalayo lombala.Umsebenzi we-Lytic wee-enzymes (umzekelo, i-Lyticase), izibulala-zintsholongwane (umz., i-Amphotericin B), kunye nohlobo lwebhaktiriya evelisa isibulala-zintsholongwane zichongiwe kusetyenziswa uvavanyo.I-anti-yeast assay iguqulelwe kwifomati ye-96-well microtiter.Zombini iimvavanyo zabonelela ngokukhawuleza, uvakalelo, kunye nokufunyanwa kwakhona kwe-lytic anti-yeast kunye nomsebenzi ochasayo wokungunda.
