I-4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Off-White Powder 98%
| Inombolo Yekhathalogu | XD90011 |
| Igama lemveliso | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| Ifomula yeemolekyuli | C12H15NO8 |
| Ubunzima beMolekyuli | 30301.25 |
| Iinkcukacha zokuGcina | -2uku-8°C |
| IKhowudi yoMrhumo eHarmonized | 29400000 |
Ukucaciswa kweMveliso
| Amanzi | <5% uKarl Fische |
| Ukunyibilika | I-1% kwi-DMF icacile kwaye ayinambala |
| Ubunyulu | I-4-Nitrophenol yasimahla <200ppm |
| HPLC | >98% |
| Imbonakalo | Umgubo omhlophe ngaphandle |
Ukuqonda komatshini kwi-Ca2 + -exhomekeke kwintsapho ye-alpha-mannosidases kwi-symbiont yamathumbu omntu.
Iibhaktheriya ze-Colonic, ezibonakaliswe yi-Bacteroides thetaiotaomicron, zidlala indima ebalulekileyo ekugcineni impilo yabantu ngokudibanisa iintsapho ezinkulu ze-glycoside hydrolases (GHs) ukuxhaphaza i-polysaccharides yokutya kunye nokusingatha i-glycans njengezondlo.Ukwandiswa kwentsapho ye-GH enjalo kubonakaliswa yintsapho ye-23 ye-GH92 glycosidases encoded yi-B. thetaiotaomicron genome.Apha sibonisa ukuba ezi zi-alpha-mannosidases ezisebenza ngendlela enye yokufuduswa ukuze kusetyenziswe i-N-glycans ebambayo.Isakhiwo sesithathu-dimensional se-GH92 mannosidases sichaza intsapho yeeprotheni ze-domain ezimbini apho i-catalytic centre ifumaneka kwi-interface yesizinda, ukubonelela nge-asidi (glutamate) kunye nesiseko (aspartate) uncedo kwi-hydrolysis kwi-Ca (2+) - indlela exhomekeke.Izakhiwo ezine-dimensional ezintathu ze-GH92 eziyinkimbinkimbi kunye ne-inhibitors zibonelela ngengqiqo kwi-speciality, indlela kunye nohambo oluhambelanayo lwe-catalysis.I-Ca(2+) idlala indima ebalulekileyo ekuncedeni ukugqwetha i-mannoside kude ne-ground-state yayo (4)C(1) i-chair conformation ngokubhekiselele kwimeko yotshintsho.（I-Bibliography: Nat.Ikhem.I-Biol.6, 125-32, (2010)
Frontal affinity chromatography of ovalbumin glycoasparagines on the concanavalin A-sepharose column.Uphononongo lobungakanani bezinto ezibophelelayo zelectin.
Ukusebenzisana kwe-Sepharose 4B-immobilized concanavalin A (ConA) kunye ne-10 glycoasparagines ephuma kwi-ovalbumin yaphandwa ngokobuninzi be-frontal affinity chromatography.Kule ndlela, isisombululo se-carbohydrate sisetyenziswa ngokuqhubekayo kwikholamu ye-ConA-Sepharose kunye nokunciphisa i-elution front ilinganiswa njengeparameter yamandla okusebenzisana.I-dissociation constant (Kd) yesaccharide nganye eneConA inokumiselwa.Uhlalutyo lokubophelela kwe-p-nitrophenyl-alpha, i-D-mannoside lubonise ukuba iipropati ezibophelelayo ze-ConA azitshintshi ngokusisiseko emva kokunyanzeliswa kwe-Sepharose 4B.Nganye ye-ovalbumin glycoasparagines ibhalwe nge-tritium yindlela yokunciphisa i-methylation yohlalutyo.Uthelekiso lwamaxabiso e-Kd afunyenweyo lubonise ukuba ukudityaniswa kwe-ConA kwahluka kakhulu nomahluko omncinci wesakhiwo setsheyini ye-glycosyl.Iziphumo zibonisa ukuba i-ConA iyaqaphela i-glycosyl chain structure, i-Man alpha 1-6 (I-Man alpha 1-3) Indoda, apho ubuncinane iqela le-hydroxyl kwi-C-3 isikhundla se-C-6-edibeneyo ye-mannose kufuneka ikhululeke.
