Ijini ye-beta-glucosidase (bgl3) evela kwiStreptomyces sp.I-QM-B814 (I-American Type Culture Collection 11238) iye yaqulunqwa ngokuncediswa kokusebenza kwe-beta-glucosidase-negative mutant ye-Streptomyces lividans.Isakhelo sokufunda esivulekileyo se-nucleotides ye-1440 ekhowuda i-polypeptide ye-479 amino acids ifunyenwe ngokulandelelana.Iprotheyini ekhowudiweyo (Bgl3) ibonisa ukufana okubanzi (ngaphezulu kwe-45% yesazisi) kunye ne-beta-glycosidases evela kwintsapho-1 glycosyl hydrolases.I-enzyme edibeneyo, ehlanjululwe ngokulandela i-ammonium sulphate precipitation kunye namanyathelo amabini e-chromatographic, i-monomeric kunye nobunzima be-molecular 52.6 kDa, njengoko kunqunywe yi-mass spectrometry, kunye ne-isoelectric point ye-pI 4.4.I-enzyme ibonakala ngathi yi-beta-glucosidase eneenkcukacha ezibanzi ze-substrate, iyasebenza kwi-cellooligomers, kwaye yenza i-transglycosylation reactions.Amaxabiso aqikelelwayo abonakalayo e-Km kwi-p-nitrophenyl-beta-D-glucopyranoside kunye ne-cellobiose yi-0.27 mM kunye ne-7.9 mM, ngokulandelanayo.Ixabiso le-Ki ye-glucose kunye ne-delta-gluconolactone, usebenzisa i-p-nitrophenyl-beta-D-glucopyranoside njenge-substrate, yi-65 mM kunye ne-0.08 mM, ngokulandelanayo.I-enzyme ecocekileyo ine-pH ye-pH eyi-6.5 kwaye elona qondo lobushushu lisebenza ngama-50 degrees.
