Iimveliso

I-Neuroblastoma, umhlaza wenkqubo ye-nervous enovelwano, lelona thumba lixhaphakileyo kwi-extracranial solid kubantwana.Ukwandiswa kwe-MYCN kunye nokunyuka kwe-BDNF / TrkB ukubonakaliswa ziimpawu ze-tumor ezinobungozi obuphezulu;ukanti, yi-˜25% kuphela yamathumba anobungozi abonisa ezi mpawu.Ke, ukuchongwa kwee-biomarkers ezongezelelweyo kunye neethagethi zonyango zibalulekile.Njengoko i-aminoacylase 1 (ACY1), i-amino acid deacetylase, i-puative tumor suppressor kwi-cell lung encinci kunye ne-renal cell carcinomas, siye saphanda ukuba ngaba okanye amanye amalungu osapho aspartoacylase (ASPA, aminoacylase 2) okanye i-aminoacylase 3 (ACY3) inokukhonza. umsebenzi ofanayo kwi-neuroblastoma.Ukubonakaliswa kwe-Aminoacylase kuhlolwe kwi-TrkB-positive, MYCN-amplified (SMS-KCNR kunye ne-SK-N-BE) kunye ne-TrkB-negative, non-MYCN-amplified (SK-N-AS, SK-N-SH, SH-SY5Y kunye SH-EP) imigca yeeseli ze-neuroblastoma.I-aminoacylase nganye ibonise indawo eyahlukileyo yendawo (okt, i-cytosolic ACY1, i-ASPA ehambelana ne-membrane kunye ne-nuclear ACY3).Xa iiseli ze-SK-N-SH ziphathwa kunye ne-neural differentiation agents (umzekelo, i-retinoic acid kunye ne-cAMP) kumajelo aqulethe i-serum ye-10%, i-ACY1 yayikuphela kwe-aminoacylase ekubonakalisweni kwayo kwalawulwa.I-ASPA yabonakaliswa ngokuyintloko kwiiseli ze-SH-EP ze-glial sublineage.I-ACY3 yachazwa kakhulu kwi-TrkB-positive, i-MYCN-amplified lines.Zontathu i-aminoacylases zibonakaliswe kwi-adrenal gland yomntu eqhelekileyo, indawo eqhelekileyo yemvelaphi ye-neuroblastoma, kodwa kuphela i-ACY1 kunye ne-ACY3 ebonisa ukubonakaliswa okubonakalayo kwi-neuroblastoma tumor.Ukumbiwa kwedatha ye-Bioinformatics yokusinda kwe-Kaplan-Meier kubonise ukuba ukubonakaliswa kwe-ACY3 ephezulu kunxulunyaniswa ne-prognosis embi, ngelixa ukubonakaliswa okuphantsi kwe-ACY1 okanye i-ASPA kuhambelana nokuxela okungalunganga.Ezi datha zibonisa ukuba intetho ye-aminoacylase i-dysregulated kwi-neuroblastoma