Iimveliso

Enye indlela ebalulekileyo yonyango kunyango lwe-2 yesifo sikashukela kukusetyenziswa kwee-agent ezinokunciphisa i-postprandial hyperglycaemia ngokuthintela i-carbohydrates digesting enzymes.Uphononongo lwangoku luphande iziphumo zesicatshulwa esikhokelwa yi-bioassay kunye namaqhezu eziqhamo ezomisiweyo ze-pericarp ye-Phaleria macrocarpa, isityalo semveli esichasene neswekile, kwi-α-glucosidase kunye ne-α-amylase, kwilinge lokuqonda indlela yabo yokulwa nesifo seswekile, njengoko kunye nesenzo sabo sokunciphisa i-postprandial glucose ukwanda.I-Methanol extract (ME), efunyenwe ngokutsalwa kwe-solvent elandelelanayo, eyona nxalenye isebenzayo ye-liquid-liquid n-butanol (NBF) kunye ne-flash column chromatographic sub-fraction (SFI), yavavanywa kwi-vitro α-glucosidase (yeast) kunye ne-α-amylase (i-porcine) inhibition yomsebenzi.Ukongezelela, ukuqinisekiswa kweemvavanyo ze-vivo zenziwa kwi-streptozotocin-induced diabetic rats (SDRs) usebenzisa i-glucose yomlomo, i-sucrose kunye neemvavanyo zokunyamezela isitashi. Kwi-concentration ephezulu eqeshwe (1 00 μg / ml), i-NBF ibonise inhibition ephezulu kwi-α-glucosidase. I-75%) kunye ne-α-amylase (87%) kwi-vitro (IC50 = 2.40 ± 0.23 μg / ml kunye ne-58.50 ± 0.13 μg / ml, ngokulandelanayo) ngendlela exhomekeke kwidosi;umphumo ofunyenwe malunga ne-20% ephezulu kune-acarbose (55%), i-α-glucosidase inhibitor eqhelekileyo (IC50 = 3.45 ± 0.19 μg / ml).I-ME kunye ne-SFI nazo zithintele i-α-glucosidase (IC50 = 7.50 ± 0.15 μg/ml kunye ne-11.45 ± 0.28 μg/ml) kunye ne-α-amylase (IC50 = 43.90 ± 0.19 μg/ml) ± 0.80 μg/ml ± 0.80ml kunye ne-6. umlinganiselo omncinci.Kwizifundo ze-vivo ezineempuku zesifo seswekile, i-NBF kunye ne-SFI yanciphisa ngempumelelo i-glucose yegazi (PBG) nge-15.08% kunye ne-6.46%, kunye nommandla ophantsi kwe-curve yokunyamezela (AUC) nge-14.23% kunye ne-12.46%, ngokulandelanayo, emva komngeni we-sucrose yomlomo. (P <0.05);ngaloo ndlela iqinisekisa isenzo se-in vitro esiqatshelweyo.Ezi ziphumo zokunciphisa kwi-PBG kunye ne-AUC nazo zibonakaliswe kwiimvavanyo ze-glucose kunye ne-starch ukunyamezela, kodwa kwinqanaba elingaphantsi.Ezi ziphumo zityhila ukuba i-P. macrocarpa inokunciphisa i-hyperglycaemia kwi-in vitro kunye neemeko ze-vivo ngokuthintela ngokukhawuleza i-carbohydrate hydrolysing enzymes, iyenze. Isityalo esisebenzayo sokufumana iikhompawundi zendalo zokulawula uhlobo lwe-2 yeswekile mellitus.