Iimveliso

Amaqela amathathu eenkawu ze-cynomolgus zamadoda amadala (i-Macaca fascicularis) zibonakaliswe nokuba yi-200 micrograms / m3 ammonium hexachloroplatinate [(NH4) 2PtCl6], i-200 micrograms (NH4) 2PtCl6 kunye ne-1 ppm ozone (O3), okanye kwi-O31 kuphela.Izilwanyana zavezwa ngokuphefumlelwa kangangeeyure ezi-6 ngosuku, iintsuku ezi-5 ngeveki kwiiveki ezili-12.Uyilo lovavanyo lubandakanya i-methacholine preexposure kunye ne-Na2PtCl6 yovavanyo lwe-bronchoprovocation, iimvavanyo zesikhumba se-Na2PtCl6, kunye ne-sera yohlalutyo lwe-antibodies.Kwiiveki ezimbini emva kokuvezwa kwe-12-wk, ezi zikhombisi ziye zavavanywa kwakhona.Umsebenzi wesiseko wemiphunga awuchaphazelekanga kakhulu ziirejimeni zokuvezwa;nangona kunjalo, ukudibanisa kwe-O3 kunye ne-NH4) 2PtCl6 yanciphisa kakhulu i-concentration ye-platinamu (Pt) ityuwa kunye ne-methacholine efunekayo ukunyusa ukuxhatshazwa kwe-pulmonary flow resistance (RL) 200% (EC200 RL).Ukuvezwa kwe-Ozone okanye i-Pt iyodwa kwakungekho mpembelelo ibalulekileyo kwezi parameters.Amaxabiso ePlatinum kunye ne-methacholine EC200 RL aye adityaniswa kakhulu kuwo omabini amaqela e-Pt-eveziweyo emva kokuvezwa.Ezi datha zibonise ukuba ukutyhileka kwe-O3 kunye ne-Pt okudityanisiweyo kuye kwanda kakhulu (Pt) kunye ne-nonspecific (methacholine) ye-bronchial hyperreactivity kaninzi kunokuba kuvezwe kwi-O3 okanye ityuwa ye-Pt yodwa.Utyhileko lwe-O3 kunye ne-Pt edityanisiweyo ikwanyusa kakhulu izehlo zovavanyo lwe-Pt olulusu xa kuthelekiswa namanye amaqela okuvezwa.Ngokufana namava omntu, uvavanyo lwe-radioallergosorbent (RAST) lwe-Pt-specific antibodies aluzange lube novakalelo njengovavanyo oluthe ngqo lwesikhumba ekuchongeni abantu abangavumiyo.