Ammonium hexachloroiridate(IV) CAS:16940-92-4
| Inombolo Yekhathalogu | XD90606 |
| Igama lemveliso | Ammonium hexachloroiridate(IV) |
| CAS | 16940-92-4 |
| Ifomula yeemolekyuli | H8Cl6IrN2 |
| Ubunzima beMolekyuli | 441.012 |
| Iinkcukacha zokuGcina | I-Ambient |
Ukucaciswa kweMveliso
| Imbonakalo | Iikristale eziluhlaza zomnquma |
| Isivavanyi | 99% |
Umonakalo owenziwe nge-oxidative kwiziseko ze-DNA udla ngokubangela ukubunjwa kwe-oxidized purines, ingakumbi i-7,8-dihydro-8-oxoguanine (8-oxoG) kunye ne-7,8-dihydro-8-oxoadenine (8-oxoA), eyayifudula iyi-well- isilonda esaziwayo se-mugenic.Ekubeni i-8-oxoG ixhomekeke ngokukhawuleza kwi-oxidation eyongezelelweyo xa kuthelekiswa neziseko eziqhelekileyo, ukufakwa kwesiseko ngexesha le-DNA synthesis ngokuchasene nefom ye-oxidized ye-8-oxoG iphandwa kwi-vitro.Itemplate yokwenziwa equlethe isilonda esisodwa se-8-oxoG yaqale yaphathwa ngee-oxidants ze-electron enye okanye phantsi kweemeko ze-oksijini ze-singlett kwaye emva koko zixhomekeke kwi-primer extension catalyzed by Klenow fragment exo- (Kf exo-), ithole le-thymus DNA polymerase alpha (pol alpha). ) okanye i-DNA yabantu yepolymerase beta (i-pol beta).Ngokuhambelana neengxelo zangaphambili, i-dAMP kunye ne-dCMP zifakwe ngokukhethayo ngokuchasene ne-8-oxoG nazo zontathu iipolymerase ze-DNA.Okubangel 'umdla kukuba, i-oxidation ye-8-oxoG yafunyaniswa ukuba ifake i-dAMP kunye ne-dGMP ngokuchasene ne-lesion yi-Kf exo- kunye nokuthintela okwethutyana ukongezwa kwe-primer o okwenzeka kwindawo yesiseko esilungisiweyo.Ngaphaya koko, isilonda senza ibhloko ngexesha lokudityaniswa kwe-DNA yi-pol alpha kunye ne-pol beta.Iimvavanyo kunye ne-8-oxoA-modified template oligonucleotide ibonisa ukuba zombini i-8-oxoA kunye nefom e-oxidized ye-8-oxoA yokufakwa ngokuthe ngqo kwe-dTMP yi-Kf exo-.Uhlalutyo olunzima lwe-spectrometric ye-8-oxoG-equlethe i-oligonucleotides ngaphambi nangemva kwe-oxidation kunye ne-IrCl62-iyahambelana ne-oxidation ye-8-oxoG indawo yokuqala, okubangelwa ukubunjwa kwe-guanidinohydantoin moiety njengemveliso enkulu.Akukho bungqina bokwenziwa kweziza ze-aasic bufunyenweyo.Ezi ziphumo zibonisa ukuba i-oxidized form ye-8-oxoG, mhlawumbi i-guanidinohydantoin, ingaqondisa ukufundwa kakubi kunye nokunganyaniseki kwe-dNTP ngexesha le-DNA synthesis.Ukuba inkqubo enjalo yenzekile kwi-vivo, iya kubonisa inqaku lesion ye-mutagenic ekhokelela kwi-G-->T kunye ne-G-->C yoguqulo.Nangona kunjalo, ifom ehambelanayo ye-oxidized ye-8-oxoA ibonisa ngokuyinhloko ukufakwa okuchanekileyo kwe-T ngexesha le-DNA synthesis kunye ne-Kf exo-.
