I-Cytidine-5'-triphosphate (CTP), ityuwa ye-disodium CAS: 652154-13-7
| Inombolo Yekhathalogu | XD90551 |
| Igama lemveliso | I-Cytidine-5'-triphosphate (CTP), ityuwa ye-disodium |
| CAS | 652154-13-7 |
| Ifomula yeemolekyuli | I-C9H14N3Na2O14P3 |
| Ubunzima beMolekyuli | 527.12 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
| Isivavanyi | 99% |
I-CTP: i-phosphocholine cytidylyltransferase α (CCTα) yi-enzyme yenyukliya eyenza inyathelo lokunciphisa izinga kwi-CDP-choline pathway for phosphatidylcholine (PC) synthesis.Ukusetyenziswa kwe-Lipid ye-CCTα kuphumela ekufudukeleni kwayo kwimvulophu yenyukliya kunye nokwandiswa kwenethiwekhi ye-intranuclear membrane ebizwa ngokuba yi-nucleoplasmic reticulum (NR) ngomatshini obandakanya ukuguqulwa kwenwebu.Iilamin zeNyukliya nazo ziyafuneka kuzinzo kunye nokwanda kwe-NR, kodwa ukuba olu lwakhiwo lulodwa, okanye i-lamina yenyukliya ngokubanzi, iyafuneka kwi-PC synthesis akwaziwa.Ukuhlolisisa olu budlelwane, i-lamina yenyukliya yachithwa yi-RNAi okanye iphazamiseke ngokubonakaliswa kwe-Hutchinson-Gilford progeria syndrome (HGPS) i-mutant lamin A (progerin), kunye nefuthe kwi-CCTα kunye ne-choline metabolism yahlaziywa.Ukuthuliswa kwe-siRNA-mediated ye-lamin A / C okanye i-lamin B1 kwiiseli ze-CHO ukunciphisa i-NR yayingenayo impembelelo kwi-PC synthesis, ngelixa i-knockdown ephindwe kabini ingathinteli ngokuthe ngqo indlela.Ukuqinisekisa t indima yakhe encinci kwi-PC synthesis, kuphela i-10% ye-choline / i-ethanolamine phosphotransferase yexesha elifutshane yafunyanwa kwi-NR.Kwiiseli ze-CHO, i-CCTα yayiyi-nucleoplasmic kwaye i-co-localized kunye ne-GFP-progerin kwiifolthi zenyukliya kunye nokuhlasela;nangona kunjalo, i-HGPS fibroblasts ibonise ukusabalalisa okungaqhelekanga kweCCTα kwi-cytoplasm kunye nemvulophu yenyukliya ehamba kunye nokunciphisa i-2-fold in PC synthesis.Naphezu kokutshintshwa kwendawo, iimvavanyo ze-choline-labeling zibonise ukuba umsebenzi we-CCT awuzange uthinteke, kwaye ukuvinjelwa kwe-PC synthesis kwalandelwa ukunciphisa umsebenzi we-hemicholinium-sensitive choline transporter.Siphetha ngelithi i-CCTα kunye neelamin zisebenzisana ngokukodwa ukwenza i-NR, kodwa ulwakhiwo lulonke lwemvulophu yenyukliya lunempembelelo encinci kumsebenzi weCCT kunye nePC synthesis.
