I-dCTP, i-2'-Deoxycytidine-5'-triphosphoric acid disodium ityuwa Cas: 102783-51-7 Umgubo oMhlophe
| Inombolo Yekhathalogu | XD90584 |
| Igama lemveliso | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium ityuwa |
| CAS | 951-78-0 |
| Ifomula yeemolekyuli | I-C9H12N2O5 |
| Ubunzima beMolekyuli | 228.20 |
| Iinkcukacha zokuGcina | -20 °C |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo oMhlophe |
| Isivavanyi | 99% |
Iiseli ze-Eukaryotic ziqulethe ibhalansi ecekethekileyo yemizuzu ye-deoxyribonucleoside triphosphates (dNTPs), eyanele kuphela imizuzu embalwa yophindaphindo lwe-DNA.Kokubini ukunqongophala kunye nentsalela ye-dNTP enye kunokubangela ukunyuka kwamazinga okuguqula, ukulungiswa kwe-DNA ephosakeleyo okanye ukuncipha kwe-DNA ye-mitochondrial.Ii-dNTP zidla ngokubalwa ngovavanyo lwe-enzymatic apho ukufakwa kwe-radioactive dATP (okanye i-radioactive dTTP kwi-assay ye-dATP) kwi-oligonucleotides yokwenziwa ethile yi-DNA polymerase ilingana nokuxinana kwe-dNTP engaziwayo.Sifumanisa ukuba i-Klenow DNA polymerase esetyenziswa ngokuqhelekileyo inokuthatha indawo ye-ribonucleotide ehambelanayo ye-dNTP engaziwayo ekhokelela kwezinye iimeko kuqikelelo olukhulu lwe-dNTPs.Ngoku sichaza iimeko zokuvavanya kwi-dNTP nganye ethintela ukufakwa kwe-ribonucleotide.Kwiimvavanyo ze-dTTP kunye ne-dATP kwanele ukunciphisa ukugxininiswa kwe-enzyme ye-Klenow kunye ne-labelled dATP (okanye i-dTTP);kwi-dCTP kunye ne-dGTP kwafuneka sitshintshe i-enzyme ye-Klenow nokuba yi-Taq DNA polymerase okanye i-Thermo Sequenase.Sicebisa ukuba kwezinye iingxelo zangaphambili i-ribonucleotide incorporation ingabangela amaxabiso aphezulu kakhulu kwi-dGTP kunye ne-dCTP.
