I-Dithizone CAS: 60-10-6 80% Black powder eluhlaza
| Inombolo Yekhathalogu | XD90464 |
| Igama lemveliso | Dithizone |
| CAS | 60-10-6 |
| Ifomula yeemolekyuli | I-C13H12N4S |
| Ubunzima beMolekyuli | 256.33 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29309098 |
Ukucaciswa kweMveliso
| Ilahleko ekomisweni | ≤5% |
| Imbonakalo | Umgubo omnyama oluhlaza |
| Isivavanyi | 80% |
| Intsalela kwi-Ignition | ≤0.2% |
Iiseli ze-Mesenchymal stem (MSCs) zichazwe njengomthombo onomtsalane wokuveliswa kweeseli ze-β ezitshintshelekayo.I-murine embryonic mesenchymal progenitor cell line C3H10T1/2 iye yaqatshelwa njengemodeli yee-MSCs, ngenxa yokukwazi ukwahlula kwemigca emininzi.Injongo yolu phononongo yayikukuphonononga ukuba ngaba iiseli ze-C3H/10T1/2 zinamandla okuhlula kwiiseli ezivelisa i-insulin (IPCs).Apha, siphande kwaye sithelekisa ulwahlulo lwe-in vitro yeempuku ze-MSCs kunye neeseli ze-C3H10T1/2 kwii-IPCs.Emva kokuba iiseli zenze ulwahlulo lwe-IPC, ukubonakaliswa kwamanqaku okuhlukanisa kwafunyanwa yi-immunocytochemistry, i-reverse transcription-polymerase chain reaction (RT-PCR), i-quantitative real-time RT-PCR (qRT-PCR) kunye ne-Western blotting.Ukukhutshwa kwe-insulin kuye kwavavanywa nge-enzyme-linked immunosorbent assay (ELISA).Ngaphaya koko, ezi seli zahlulwe zatshintshwa zibe yi-streptozotocin-induced diabetic mice kwaye imisebenzi yazo yebhayoloji yavavanywa kwi-vivo.Olu pho nonongo lubika indlela ye-2-inqanaba lokuvelisa ii-IPCs kwiiseli ze-C3H10T1/2.Ngaphantsi kweemeko ezithile zokungeniswa kweentsuku ze-7-8, iiseli ze-C3H10T1 / 2 zenze imizimba ye-spheroid emithathu-dimensional (SBs) kunye ne-insulin eyimfihlo, ngelixa isizukulwana se-IPC esivela kwi-rat MSCs sifuna ixesha elide (ngaphezu kweeveki ze-2).Ngaphezu koko, ezi IPCs zithathwe kwiiseli ze-C3H10T1 / 2 zafakwa kwiigundane zesifo sikashukela kwaye ziphucula i-basal glucose, ubunzima bomzimba kunye novavanyo oluqhelekileyo lokunyamezela i-glucose.Uphononongo lwangoku lubonelele ngemodeli elula nethembekileyo ye-vitro yokuphanda ngakumbi indlela ephantsi kwe-IPC yokwahlulwa kwe-MSCs kunye nonyango lokubuyisela iiseli kwisifo seswekile.
