Iimveliso

Kweli phepha, i-pH-inductive protein-scaffold biosynthesis ye-shape-tunable crystalline gold nanoparticles kwiqondo lobushushu begumbi iye yaphuhliswa.Ngokuphathwa ngokulula kwesisombululo se-pH yesisombululo, i-anisotropic nanoparticles zegolide eziquka ingqukuva, oonxantathu kunye neetyhubhu zinokuveliswa ngokufukamela isisombululo esimanzi se-sodium tetrachloroaurate nge-Dolichomitriopsis diversiformis biomasses emva kokuntywiliselwa emanzini e-ultrapure eMillipore ngobusuku.Iprotheyini yobulembu enobunzima bemolekyuli malunga ne-71 kDa kunye ne-pI ye-4.9 yayiyeyona nto iphambili ye-biomolecule ebandakanyekayo kwi-biosynthesis yeenanoparticles zegolide.Ukucwangciswa kwesibini kweeprotheni nge-CD spectrum kuthetha ukuba iprotheni ye-moss ingabonisa ukucwangciswa kwesibini okuhlukeneyo kuquka i-coil engahleliweyo, i-α-helix kunye ne-intermediate conformations phakathi kwe-coil engahleliwe kunye ne-α-helix kwisisombululo se-pH sovavanyo.Inkqubo yokukhula ye-nanoparticles yegolide ibonise ngakumbi ukuba iprotein ye-moss enobume obahlukeneyo ibonelele i-template sc affold ye-biosynthesis elawulwa yimilo yeenanoparticles zegolide.Ubume obunyanzelwayo be-nanoparticles yegolide, nangona kunjalo, wanyamalala kwisicatshulwa sobulembu obubilisiweyo.I-nanoparticles yegolide ene-morphology eyilwe yaphinda yakhiwa ngempumelelo kusetyenziswa iprotein ye-moss ehlanjululwe kwiinanoparticles zegolide.Iimpawu zokwakheka kwe-SEM, i-TEM kunye ne-SAED zibonise ukuba i-triangular kunye ne-cubic nanoparticles yegolide yayiyikristale enye.