I-Guanidine Hydrochloride Cas: 50-01-1 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90198 |
| Igama lemveliso | Guanidine Hydrochloride |
| CAS | 50-01-1 |
| Ifomula yeemolekyuli | I-CH6N3Cl |
| Ubunzima beMolekyuli | 95.5388 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29252900 |
Ukucaciswa kweMveliso
| Indawo yokunyibilika | 185.3 - 187.5 Deg C |
| Amanzi | <0.3% |
| Ukunyibilika | Sula isisombululo esingenambala kwi-6M emanzini |
| Isivavanyi | >99% |
| Ukungabikho kweUV | 0.10 kwi-260nm |
| Imbonakalo | Umgubo omhlophe |
| Isixhobo se-IR | Iyahambelana |
Enye yezinto ezithintela ukusetyenziswa kweklinikhi yeyeza lokuvuselela kwizifo ze-cartilage eziphazamisayo ngumthombo ofanelekileyo weeseli.I-Chondrocytes, uhlobo olulodwa lweeseli ze-cartilage, ezikhule kwi-vitro phantsi kweemeko zenkcubeko ukwandisa amanani eeseli zilahlekelwa yi-phenotype yazo kunye nokukwazi ukuvelisa i-hyaline cartilaginous tissue.Kule sifundo sinquma ukuba inkqubo yenkcubeko ye-serum kunye nokukhula-free-factor-free-dimensional (3D) ibuyisela amandla e-chondrocytes adlulisiweyo ukwenza i-cartilage tissue in vitro, inkqubo ebandakanya i-sox9.I-Bovine articular chondrocytes yagqithiswa kabini ukuya vumela ukwandiswa kwenombolo yeseli (P2) kwaye ikhuliswe kwi-high density kwi-3D i-collagen-type-II-coated membranes kwi-high glucose content media eyongezwe nge-insulin kunye ne-dexamethasone (SF3D).Iiseli zibonakaliswe emva kokwandiswa kwe-monolayer kunye nokulandela inkcubeko ye-3D ngokuhamba kwe-cytometry, i-gene expression, kunye ne-histology.Utshintsho lwangaphambili kwiindlela zokutshintshwa kweempawu ngexesha le-ion ye-redifferentiat zibonakaliswe.Iiseli ze-P2 zibonise iphrofayili ye-antigen efana ne-99% ye-CD44 (+) kunye ne-40% ye-CD105 (+) kunye neprofayili ye-gene ebonisa i-interzone cells.I-P2 kwi-SF3D ivakalise iijene ze-chondrogenic kunye ne-matrix ye-extracellular eqokelelweyo.Ukunciphisa i-insulin receptor (IR) kunye ne-HNMPA- (AM3) okanye i-PI-3 / AKT kinase indlela (eyenziwe ngonyango lwe-insulin) kunye ne-Wortmannin inhibited collagen synthesis.I-HNMPA-(AM3) iyancipha intetho ye-Col2, i-Col11, kunye ne-IR yofuzo kunye ne-Sox6 kunye ne-9.I-Co-immunoprecipitation kunye ne-chromatin immunoprecipitation analysis of HNMPA-(AM3)-treated cells ibonise ukubopha i-coactivators i-Sox6 kunye ne-Med12 kunye ne-Sox9 kodwa iyancipha i-Sox9-Col2a1 yokubopha.Sichaza indlela yenkcubeko inoveli evumela ukwanda kwenani le-chondrocytes kwaye ikhuthaza. i-hyaline-like cartilage tissue formation in part by insulin-mediated Sox9-Col2a1 binding.Ukufaneleka kwezicubu eziveliswe ngale ndlela ukuze zisetyenziswe ekulungiseni ngokubambisana kufuneka kuhlolwe kwi-vivo.
