ITP, inosine 5′-triphosphate trisodium ityuwa
| Inombolo Yekhathalogu | XD90558 |
| Igama lemveliso | ITP, inosine 5'-triphosphate trisodium ityuwa |
| CAS | 35908-31-7 |
| Ifomula yeemolekyuli | I-C10H12N4Na3O14P3 |
| Ubunzima beMolekyuli | 574.111 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
| Isivavanyi | 99% |
I-infrared spectroscopy isetyenziselwe ukwenza imephu yokusebenzisana kwe-substrate-protein: utshintsho oluhambelanayo lwe-sarcoplasmic reticulum Ca (2+) -ATPase phezu kokubopha i-nucleotide kunye ne-ATPase phosphorylation ibekwe esweni kusetyenziswa i-substrate ATP kunye ne-ATP analogue (2'-deoxy-ATP, 3 '-deoxy-ATP, kunye ne-inosine 5'-triphosphate), eziye zatshintshwa kumaqela athile asebenzayo e-substrate.Ukuguqulwa kwe-2'-OH, i-3'-OH, kunye neqela le-amino le-adenine linciphisa ubungakanani bokuguqulwa kwe-conformational-induced conformational ye-ATPase, kunye neziphumo ezinamandla ngokukodwa eziqatshelweyo ezimbini zokugqibela.Oku kubonisa ubuntununtunu besakhiwo se-nucleotide-ATPase eyinkimbinkimbi kwintsebenziswano yomntu ngamnye phakathi kwe-nucleotide kunye ne-ATPase.Onke amaqela afundwayo abalulekile ekubopheni kunye nokusebenzisana kweqela elinikwe i-ligand kunye ne-ATPase kuxhomekeke ekusebenzisaneni kwamanye amaqela e-ligand.I-phosphorylation ye-ATPase yabonwa kwi-ITP kunye ne-2'-deoxy-ATP, kodwa kungekhona i-3'-deoxy-ATP.Akukho nxu lumano oluthe ngqo phakathi komlinganiselo wenguqu ehambelanayo kwi-nucleotide ebophelelayo kunye nesantya se-phosphorylation ebonisa ukuba ubungakanani obupheleleyo be-ATP-induced conformational change ayinyanzelekanga kwiphosphorylation.Njengoko kuqwalaselwe i-nucleotide-ATPase complex, ukulungelelaniswa kwe-phosphorylated ATPase yokuqala ephakathi kwe-E1PCa (2) nayo ixhomekeke kwi-nucleotide, ebonisa ukuba i-ATPase ithi i-ATPase ine-conformation encinci ye-uniform kunokuba bekulindelwe ngaphambili.
