L-Asparagine anhydrous CAS: 70-47-3 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90314 |
| Igama lemveliso | D-Aspartic acid |
| CAS | 70-47-3 |
| Ifomula yeemolekyuli | C4H8N2O3 |
| Ubunzima beMolekyuli | 132.12 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29241900 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
| Isivavanyi | 99% |
| Ukujikeleza okuthe ngqo | +34,2 ukuba +36,5 |
| Iintsimbi ezinzima | <10ppm |
| AS | <1ppm |
| pH | 4.4 - 6.4 |
| SO4 | <0.020% |
| Fe | <10ppm |
| Ilahleko ekomisweni | <0.5% |
| NH4 | <0.10% |
| Cl | <0.020% |
| Imeko yesisombululo | >98% |
I-MSMEG_0307 ichazwa njengolawulo olushicilelweyo losapho lweprotein ye-AraC kwaye ibekwe kufutshane nejini ye-arylamine N-acetyltransferase (nat) kwiMycobacterium smegmatis, kwiqela lemfuza, elondolozwe kwiintlobo ezininzi ze-mycobacterial yokusingqongileyo.Ukuze kucaciswe umsebenzi weprotheyini ye-AraC evela kwi-nat operon kwi-M. smegmatis, ii-palindromic DNA motifs ezimbini ezigciniweyo zachongwa kusetyenziswa i-bioinformatics kwaye zavavanyelwa iprotheyini ebophelelayo kusetyenziswa i-electrophoretic mobility shift assays kunye ne-recombinant form ye-protein ye-AraC.Sichonge ukubunjwa kwe-DNA: i-protein ye-AraC ene-protein ene-motifs kunye nobukho be-motif kwi-loci ye-20 kuyo yonke i-genome ye-M. smegmatis, exhasa ubukho be-AraC elawulwa yi-regulon.Ukubonakalisa imiphumo ye-AraC kulawulo lwe-nat operon genes, kunye nokufumana ingqiqo engakumbi kumsebenzi wayo, senze i-ΔaraC ye-mutant strain apho i-araC gene yatshintshwa yi-hygromycin resi stance marker.Inqanaba lokubonakaliswa kwe-nat kunye ne-MSMEG_0308 yofuzo yayilawulwa phantsi kwi-ΔaraC strain xa ithelekiswa nohlobo lwasendle olubonisa ifuthe le-activator ye-AraC protein ekubonakalisweni kwe-nat operon gene.
