Iimveliso

I-EAAT2 glutamate transporter, i-akhawunti ye>90% ye-hippocampal glutamate uptake.Nangona i-EAAT2 ibonakaliswa ikakhulu kwii-astrocytes, ~i-10% yeemolekyuli ze-EAAT2 zifumaneka kwiitheminali ze-axon.Ngaphandle kwenqanaba elisezantsi le-EAAT2 yokubonisa kwii-terminals ze-glutamatergic, xa izilayi ze-hippocampal zifukanywe ngoxinaniso oluphantsi lwe-d-aspartate (i-EAAT2 substrate), iitheminali ze-axon ziqokelela i-d-aspartate ngokukhawuleza njenge-astroglia.Oku kuthetha ukungangqinelani okungachazwanga phakathi kokusasazwa kweprotheyini ye-EAAT2 kunye nomsebenzi wothutho olawulwa yi-EAAT2.Enye ingqikelelo yeyokuba (1) utshintshiselwano nge-heteroexchange ye-substrate yangaphakathi kunye ne-substrate yangaphandle ikhawuleza kakhulu kunokuthathwa komnatha kwaye (2) ii-terminals zithanda ukutshintshiselana nge-heteroexchange ngenxa yamanqanaba aphezulu e-glutamate yangaphakathi.Nangona kunjalo, okwangoku akwaziwa ukuba i-heteroexchange kunye nokuthatha kunemilinganiselo efanayo okanye eyahlukileyo.Ukujongana nalo mbandela, sisebenzise inkqubo ehlaziyiweyo ukuthelekisa amaxabiso ahambelanayo kwiinkqubo ezimbini kwiigundane kunye neempuku.I-Net uptake ibonakaliswe kutshintsho kwi-membrane enokwenzeka kwaye yavuselelwa yi-anion yangaphandle ekwazi ukungena ngemvume kunye nobukho be-anion conductance engadityaniswanga.Ngokusebenzisa le yokugqibela, sikwabonisa ukuba izinga le-heteroexchange likwaxhomekeke kwi-membrane enokubakho.Ukongezelela, idatha yethu iphakamisa ubukho bokuvuza kwe-sodium kwi-EAAT2.Ngokubandakanya izinto ezintsha ezifunyenweyo kwimodeli yethu yangaphambili yokuthatha i-glutamate yi-EAAT2, siqikelela ukuba uvakalelo lombane lokutshintshiselana lubangelwa kukubophelela kwe-voltage yesithathu exhomekeke kwi-Na(+).Ngaphaya koko, yomibini imifuniselo yethu kunye nokulinganisa kucebisa ukuba amaxabiso anxulumeneyo okufumana kunye ne-heteroexchange ayathelekiseka kwi-EAAT2.