I-L-Cystine CAS: 56-89-3 99% Iikristale ezimhlophe okanye i-crystalline powder
| Inombolo Yekhathalogu | XD90322 |
| Igama lemveliso | L-cystine |
| CAS | 56-89-3 |
| Ifomula yeemolekyuli | I-C6H12N2O4S2 |
| Ubunzima beMolekyuli | 240.30 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29309013 |
Ukucaciswa kweMveliso
| Imbonakalo | Iikristale ezimhlophe okanye i-crystalline powder |
| Isivavanyi | 99% |
| IBanga | USP32 |
| Ukujikeleza okuthe ngqo | -215 ° ukuya -225 ° |
| Iintsimbi ezinzima | <0.0015% |
| AS | 1.5ppm ubuninzi |
| SO4 | 0.040% ubuninzi |
| Fe | <0.003% |
| Ilahleko ekomisweni | 0.20% ubuninzi |
| Intsalela kwi-Ignition | 0.10% ubuninzi |
| Cl | 0.10% ubuninzi |
Ulwakhiwo lwekristale ye-carboxypeptidase T (CpT) ekhompleksini ene-phenylalanine kunye ne-arginine substrate analogs - benzylsuccinic acid kunye (2-guanidinoethylmercapto) succinic acid - zigqitywe yindlela yokutshintshwa kwemolekyuli kwizisombululo ze-1.57 Å kunye ne-1.62 yeprofayile ebanzi ukucacisa iprofayile ethile. kwi-enzyme.I-Conservative Leu211 kunye neentsalela zeLeu254 (nakhona zikhoyo kuzo zombini i-carboxypeptidase A kunye ne-carboxypeptidase B) ziboniswe njengezimiselo zolwakhiwo lokuqatshelwa kwe-hydrophobic substrates, kanti i-Asp263 yayiyeyokuqatshelwa kwe-substrates ehlawulwe kakuhle.Ukuguqulwa kwezi zigqibo kuguqula iprofayili ye-substrate: i-CPT eyahlukileyo ye-Leu211Gln ifumana iimpawu ezifana ne-carboxypeptidase B, kunye ne-CpT eyahlukileyo ye-Asp263Asn i-carboxypeptidase A-efana nokukhetha.I-loop ye-Pro248-Asp258 esebenzisana ne-Leu254 kunye ne-Tyr255 iboniswe inoxanduva lokuqaphela intsalela ye-C-terminal ye-substrate.Ukubophelela kwe-Substrate kwi-subsite ye-S1 kukhokelela ekutshintsheni kwe-ligand-dep yale loop, kwaye i-Leu254 ye-side chain movement ibangela ukulungelelaniswa kwakhona kwe-Glu277 intsalela ebalulekileyo kwi-catalysis.Olu lwazi lunoveli kwi-substrate selectivity ye-metallocarboxypeptidases ebonisa ukubaluleka kobudlelwane phakathi kwe-subsite ye-S1 kunye neziko le-catalytic.
