L-lysine dihydrochloride Cas: 657-26-1 99% Umgubo omhlophe wekristale
| Inombolo Yekhathalogu | XD90307 |
| Igama lemveliso | L-lysine dihydrochloride |
| CAS | 657-26-1 |
| Ifomula yeemolekyuli | C6H14N2O2.2[HCl] |
| Ubunzima beMolekyuli | 219.10944 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29224100 |
Ukucaciswa kweMveliso
| Indawo yokunyibilika | 200 - 205 Deg C |
| Amanzi | <0.5% |
| Ukujikeleziswa kwe-Optical | +15,9 |
| Imbonakalo | Umgubo omhlophe wekristale |
| Uvavanyo (Titration ex chloride) | >99% |
Ukuqokelelana kweNyukliya kohlobo oluguqukileyo lweprotein yenyukliya iLamin-A, ebizwa ngokuba yiProgerin (PG) okanye iLamin AΔ50, kwenzeka kwiHutchinson-Gilford Progeria Syndrome (HGPS) okanye iProgeria, isifo sokuguga esikhawulezayo.Enye yeempawu eziphambili zolu kuphazamiseka kwemfuza kukulahlekelwa ngamafutha angaphantsi kwesikhumba ngenxa ye-lipodystrophy ephawulekayo.Wenze ngokuzinzileyo ukubonakaliswa kwePG yabantu kunye ne-GFP -iProtein yeFluorescent eluhlaza-njengolawulo kwiiseli ze-3T3L1 usebenzisa inkqubo ye-lentviral ukufunda isiphumo sokubonakaliswa kwePG kumthamo wokwahlula lo mgca weseli, enye yeemodeli ezisetyenziswa kakhulu ze-adipogenic.Iiproteomics zobungakanani (i-iTRAQ) zenziwe ukufunda isiphumo sokuqokelelwa kwePG.Uninzi lweeprotheni ezimodareyithiweyo zaqinisekiswa nge-immunoblotting kunye ne-PCR yexesha langempela.Umsebenzi weMitochondrial uhlalutywe ngokulinganisa a) umsebenzi wesiseko we-mitochondrial, b) ukuveliswa kwe-anion ye-superoxide kunye c) nokusebenza komntu ngamnye kobunzima obuhlukeneyo bekhonkco lokuphefumla. ukwehla kwezinga lokukhula kunye neziphene kumthamo we-adipogenic xa kuthelekiswa nolawulo.Uhlalutyo lweproteomics yobuninzi lubonise iiprotheyini ze-181 kakhulu (p <0.05) ezimodareyithwe kwi-PG-ebonisa i-preadipocytes.Umsebenzi weMitochondrial wonakele kwiiseli ezibonisa i-PG.Ngokukodwa, siye safumanisa ukwanda komsebenzi we-complex I kunye nokuveliswa kwe-Superoxide anion.I-Incubation kunye ne-Reactive Oxygen Species (ROS) i-scavenger agents iqhubela ekunciphiseni kwe-autophagic proteolysis njengoko kutyhilwa yi-LC3-II/LC3-I ratio.PG ukubonakaliswa kwiiseli ze-3T3L1 kukhuthaza utshintsho kwiiNkqubo ezininzi ze-Biological, kuquka isakhiwo se-cytoskeleton, i-lipid metabolism, i-calcium. ulawulo, uguqulo, ukusonga iiprotheyini kunye nokuveliswa kwamandla yi-mitochondria.Idatha yethu yomeleza igalelo le-ROS yokuqokelela kwi-phenotype yokuguga ngaphambi kwexesha kwaye iseke ikhonkco phakathi kokungasebenzi kakuhle kwe-mitochondrial kunye nokulahleka kweproteostasis kwi-HGPS.
