I-L-Methionine Cas: 63-68-3 99% I-crystalline powder emhlophe
| Inombolo Yekhathalogu | XD90287 |
| Igama lemveliso | L-Methionine |
| CAS | 63-68-3 |
| Ifomula yeemolekyuli | I-C5H11NO2S |
| Ubunzima beMolekyuli | 149.21134 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29304010 |
Ukucaciswa kweMveliso
| Isivavanyi | 98% - 101.5% |
| Imbonakalo | Iikristale ezimhlophe okanye i-crystalline powder |
| Ukuqukumbela | |
| Ukuchongwa | Iyahlangabezana nemfuneko |
| pH | 5.6 - 6.1 |
| Ilahleko ekomisweni | ≤ 0.3% |
| Isulfate (SO4) | ≤ 0.03% |
| Intsimbi | ≤ 0.003% |
| Intsalela kwi-Ignition | ≤ 0.4% |
| I-Chloride | ≤ 0.05% |
| Intsimbi enzima | ≤ 0.0015% |
| Ukucoceka kweChromotograhic | Akukho ngaphezu kwe-2.0% yokungcola okupheleleyo kufunyenwe |
| Ukujikeleza ngokuthe ngqo [ α ] D 2 5 | +22.4º ~ +24.7º |
Uphononongo lwethu lwangaphambili luveze indima yalo ebalulekileyo kumanqanaba okuqala kunye nasemva kwexesha losulelo lwe-KSHV.Kolu phononongo, siphonononge i-interantome ye-ORF45 sisebenzisa ipaneli ye-monoclonal antibodies.Ukongeza kwiiprotheni ezichongiweyo ze-extracellular regulated kinase (ERK) kunye ne-p90 ribosomal S6 kinase (RSK), sifumene ezinye iiprotheni ezininzi ezidibeneyo, ezibandakanya ezibalaseleyo ze-~38 kDa kunye ne-130 kDa.I-mass spectrometry ibonise ukuba iprotheni ye-38-kDa yintsholongwane ye-ORF33 kunye neprotheni ye-130-kDa yi-USP7 yeselula (ubiquitin-specific protease 7).Senze imephu ye-ORF33-binding domain kwi-carboxyl-terminal 19 amino acids (aa) egcinwe kakhulu ye-ORF45 kunye ne-USP7-binding domain kwi-motif yesivumelwano esixeliweyo kumbindi wengingqi ye-ORF45.Ukusebenzisa i-immunofluorescence staining, siye saqwalasela i-colocalization ye-ORF45 kunye ne-ORF33 okanye i-USP7 zombini phantsi kweemeko ezosulelekileyo kunye neeseli ezosulelwe yi-KSHV.Ngaphezu koko, siqaphele ukutshintshwa kwe-ORF45 exhomekeke kwinxalenye ye-ORF33/USP7 ukusuka kwi-nucleus ukuya kwi-cytoplasm.Sifumene ukuba i-ORF45 ibangele ukwanda kwe-ORF33 yeprotheyini yokuqokelela yapheliswa ukuba mhlawumbi i-ORF33- okanye i-USP7-binding domain kwi-ORF45 yacinywa.Ngapha koko, ukucinywa kwe-carboxyl terminus ye-ORF45 kwi-genome ye-KSHV kunciphise kakhulu inqanaba leprotein ye-ORF33 kwiiseli ezosulelwe yi-KSHV kunye nokuphelisa ukuveliswa kwee-virions zenzala.Ngokudibeneyo, iziphumo zethu azivezi kuphela amacandelo amatsha e-ORF45 interactome, kodwa zikwabonisa ukuba intsebenziswano phakathi kwezi proteni ibalulekile kwi-KSHV lytic replication.I-Kaposi's sarcoma-associated herpesvirus (KSHV) yi-causative agent yee-cancer ezininzi zabantu.I-KSHV ORF45 yiprotheni esebenzayo efunekayo kwi-KSHV lytic replication, kodwa iindlela ezichanekileyo i-ORF45 eyenza ngayo imisebenzi yayo ebalulekileyo ayicacanga.Uphononongo lwethu lwangaphambili lubonise ukuba zonke iiprotheyini ze-ORF45 kwiiseli zikhona kwii-high-molecular-weight complexes.Ke ngoko siye sazama ukubonisa unxibelelwano lwe-ORF45 ukubonelela nge-insights kwiindima zayo ngexesha lokuphindaphinda kwe-lytic.Ukusebenzisa ipaneli ye-antibodies ye-monoclonal, sihlolisise i-ORF45 interactome kwiiseli ezosulelwe yi-KSHV.Sichonge amaqabane amabini amatsha abophezelayo e-ORF45: iprotheni yentsholongwane ye-ORF33 kunye ne-cellular ubiquitin-specific protease 7 (USP7).Sibonisa ngakumbi ukuba intsebenziswano phakathi kwe-ORF45 kunye ne-ORF33 ibalulekile kwimveliso esebenzayo yeentsholongwane ze-KSHV zentsholongwane, ebonisa ukuba ukuphazamiseka okujoliswe kuyo kolu nxibelelwano kunokubonisa isicwangciso senoveli ukuvimbela ukuphindaphinda kwe-KSHV lytic.
