I-L-Proline Cas: 147-85-3 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90293 |
| Igama lemveliso | L-Proline |
| CAS | 147-85-3 |
| Ifomula yeemolekyuli | C5H9NO2 |
| Ubunzima beMolekyuli | 115.13046 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29339980 |
Ukucaciswa kweMveliso
| Isivavanyi | 99% min |
| Imbonakalo | Umgubo omhlophe |
| Ukujikeleza okuthe ngqo | -84.5 ukuya -86 |
| Iintsimbi ezinzima | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Ilahleko ekomisweni | <0.3% |
| Intsalela kwi-Ignition | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Imeko yesisombululo | >98% |
Ukuqonda i-metabolism ye-microbial host kubalulekile kuphuhliso kunye nokuphucula iinkqubo ze-biocatalytic ezisekelwe kwiseli epheleleyo, njengoko iyalela ukusebenza kakuhle kwemveliso.Oku kuyinyani ngakumbi kwi-redox biocatalysis apho iiseli ezisebenzayo zemetabolism ziqeshwe ngenxa ye-cofactor/cosubstrate regenerative capacity endogenous kumamkeli.I-Recombinant Escherichia coli yayisetyenziselwa ukuvelisa ngokugqithiseleyo i-proline-4-hydroxylase (P4H), i-dioxygenase catalyzing i-hydroxylation ye-L-proline yamahhala kwi-trans-4-hydroxy-L-proline kunye ne-ketoglutarate (a-KG) njenge-cosubstrate.Kulo lonke iseli ye-biocatalyst, i-carbon metabolism ephakathi ibonelela nge-cosubstrate a-KG efunekayo, ukudibanisa ukusebenza kwe-P4H ye-biocatalytic ngokuthe ngqo kwi-carbon metabolism kunye nomsebenzi we-metabolic.Ngokusebenzisa izixhobo zebhayoloji zokulinga kunye nezokubala, ezifana nobunjineli bemetabolism kunye (13) nohlalutyo lwe-C-metabolic flux ((13) C-MFA), siphande kwaye sachaza ngokobungakanani impendulo ye-physiological, metabolic, kunye ne-bioenergetic ye-cell-cell biocatalyst. kuguqulo lwebhayoloji ekujoliswe kulo kwaye kwachongwa imiqobo yemetabolism enokwenzeka ngakumbi kubunjineli bendlela enengqiqo.Uhlobo lokuthotywa kweproline olunqongopheleyo lwe-E. coli lwakhiwa ngokucima i-puA gene encoding proline dehydrogenase.I-biotransformations yeeseli ezipheleleyo ezinolu hlobo oluguqukileyo alukhokelelenga kuphela kubungakanani beproline hydroxylation kodwa nokuphinda-phindeka kabini kweqondo elithile lokubunjwa kwe-trans-4-L-hydroxyproline (hyp), xa kuthelekiswa nohlobo lwasendle.Uhlalutyo lwe-carbon flux ngokusebenzisa i-metabolism ephakathi ye-mutant strain ibonise ukuba ukunyuka kwe-KG yemfuno ye-P4H ayizange ikhulise i-a-KG evelisa i-flux, ebonisa ukusebenza komjikelo olawulwa ngokuqinileyo we-TCA phantsi kweemeko ezifundiweyo.Kuhlobo lwasendle, i-P4H synthesis kunye ne-catalysis yabangela ukunciphisa isivuno se-biomass.Okubangela umdla kukuba, uhlobo lwe-ΔputA luphinde lwabuyekeza ilahleko ye-ATP kunye ne-NADH ehambelanayo ngokunciphisa iimfuno zamandla olondolozo kumazinga aphantsi aphantsi okufunxa iswekile, endaweni yokonyusa umsebenzi we-TCA. ube nethemba lokuvelisa i-P4H catalysis kungekuphela nje ngokwemveliso ye-biotransformation, kodwa malunga namazinga e-biotransformation kunye ne-proline uptake kunye nesivuno se-hyp kumthombo wamandla.Iziphumo zibonisa ukuba, xa i- putA knockout, udibaniso lomjikelo we-TCA kwi-proline hydroxylation nge-cosubstrate a-KG iba ngumba ongundoqo onyanzelisayo kunye nokujoliswe kuko ekuphuculeni ngakumbi ukusebenza kakuhle kwe-a-KG-exhomekeke kwi-biotransformations.
