Iimveliso

Kwindlela ye-kinetic angiotensin-converting enzyme (ACE), isithinteli esisebenzayo kunye nesifanelekileyo yi-80 mmol / L ye-borate buffer kwi-pH 8.2 (37 degrees C).Isigaba se-lag sifunyenwe kwi-reaction, kwaye i-5-min incubation ye-substrate kunye ne-plasma icetyiswa ngaphambi kokulinganisa i-kinetic.I-substrate, i-N- [3- (2-furyl) acryloyl] -L-phenylalanylglycylglycine (FAPGG), i-concentration inyuswe kwi-1.0 mmol / L kunye ne-wavelength yokulinganisa i-345 nm ukuqinisekisa umgca wokulinganisa.Inkqubo ecetywayo isebenzisa i-1: 9 i-plasma-to-reagent volume ratio.Uluhlu lomgca we-assay lufikelela malunga ne-170 U / L, emele i-25% ye-substrate hydrolysis.I-FAPGG i-absorptivity inqunywe ngokulinganisa umahluko phakathi kwe-1.0 mmol / L FAPGG kunye nezisombululo zemveliso.Ukunyaniseka kwe-wavelength kuhlolwe ngokuqaphela ixabiso elilindelekileyo lokuxhamla kwisisombululo se-FAPGG, kunye nokuphambuka kwe-1.0-nm ukusuka kwi-345 nm kuguqula i-absorbence nge-15.5%.Ukuchaneka kweemvavanyo ze-ACE malunga ne-60 kunye ne-100 U / L yi-3.5% kunye ne-2.4% ngaphakathi kwebhetshi kunye ne-2.9% kunye ne-2.6% phakathi kwebhetshi, ngokulandelanayo.Ixesha lokubhekisela (i-2.5th ukuya kwi-97.5th percentiles) yi-41-139 U / L, kwaye akukho mahluko phakathi kwamaxabiso kumadoda nabasetyhini.