I-NSP-SA-NHS CAS: 199293-83-9 I-crystalline powder ephuzi
| Inombolo Yekhathalogu | XD90129 |
| Igama lemveliso | I-NSP-SA-NHS |
| CAS | 199293-83-9 |
| Ifomula yeemolekyuli | I-C32H31N3O10S2 |
| Ubunzima beMolekyuli | 681.733 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo wekristale otyheli |
| Isivavanyi | 99% |
I-Acridine ester (NSP-SA-NHS) i-Cas199293-83-9 kunye neekhompawundi zayo ezihambelanayo ziluncedo kakhulu iilebhile ze-chemiluminescent ezizinzo, umsebenzi kunye nobuntununtunu budlula ezinye ii-radioisotopes.I-Acridine esters inokusabela kunye neeprotheni eziqukethe amaqela aphambili e-amino.Ngaphantsi kweemeko ezisisiseko, i-NHS ithathelwa indawo njengeqela elishiyayo, kwaye iprotheni yenza i-amide bond ezinzileyo kunye ne-acridine ester.Emva kokuba impendulo igqityiwe, ityuwa ye-acridinium engaphezulu yasuswa ngekholomu ye-dessalting.
Iiprotheni ezibhalwe nge-Acridine azifuni i-enzymatic catalysis ukuze zikhuphe ukukhanya phambi kwe-alkaline peroxide ye-hydrogen.Umgaqo ocacileyo wokukhutshwa kokukhanya kukuba kwisisombululo se-alkaline ye-hydrogen peroxide, i-acridine ester ihlaselwa yi-hydrogen peroxide ion ukuvelisa i-dioxyethane engazinzanga kunye noxinzelelo, oluye lwachithwa ngakumbi kwi-CO2 kunye ne-electronic acridone enomdla.Xa i-acridone ibuyela kumhlaba womhlaba, ikhupha iifotoni ezinobuninzi be-absorption wavelength ye-430 nm.Le nkqubo ye-luminescence imfutshane kakhulu (inkqubo yonke ithatha ngaphantsi kwemizuzwana emi-2), kwaye isicwangciso sokuqalisa kufuneka songeze i-photometer yangaphakathi kunye ne-photon detector;Ukongeza, le mveliso inokusebenzisa i-multi-function microplate reader exhotyiswe nge-autosampler yokuqokelela idatha ye-luminescence.Iiprotheyini, iipeptides, amajoni omzimba, kunye neenucleic acids zonke zinokubhalwa ngale mveliso.I-acridine esters ikhupha ukukhanya ngokukhawuleza phantsi kwe-excitation ye-alkaline hydrogen peroxide, ngoko ke iikhompawundi ezibhaliweyo zingabonwa ngokuqokelela iifotoni.
Le mveliso isetyenziselwa kakhulu: i-chemiluminescence kunye ne-immunoassay, uhlalutyo lwe-receptor, i-nucleic acid kunye nokufumanisa i-peptide kunye nolunye uphando.
