I-Penicillin G ityuwa ye-potassium CAS: 113-98-4 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90351 |
| Igama lemveliso | I-Penicillin G yetyuwa ye-potassium |
| CAS | 113-98-4 |
| Ifomula yeemolekyuli | I-C16H17KN2O4S |
| Ubunzima beMolekyuli | 372.48 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29411000 |
Ukucaciswa kweMveliso
| Ilahleko ekomisweni | <1.0% |
| Umbala wesisombululo | Ayinambala kakhulu kunokuqina Y2 okanye YG2 yoluhlu lwereferensi |
| pH | 5-7.5 |
| Izinto ezinxulumeneyo | <1.0% |
| Isivavanyi | 99% |
| Amandla | 1440 - 1680u/mg |
| Imbonakalo | Mhlophe ukuya phantse kumhlophe crystalline umgubo |
| Ukucaca kwesisombululo | Akukho ngaphezu kwe-opalescent kunokumiswa kwereferensi 1 |
| Ugqithiso | NLT 90% |
| Butyl acetate | NMT 0.05% |
| I-Butanol | NMT 0.12% |
e ichazwe ngaphambili ukuba i-molecule ye-autoinducer, echongiweyo njenge-1,3-diaminopropane (1,3-DAP), ikhutshwe yiPenicillium chrysogenum kunye ne-Acremonium chrysogenum.Ukusebenzisa iinkcubeko zefermentor ezilawulwa yi-pH esizibonile kulo msebenzi ukuba i-1,3-DAP kunye ne-spermidine ivuselela ngokucacileyo i-biosynthesis ye-benzylpenicillin kwi-P. chrysogenum, zombini kwi-media echazwe kunye neyinkimbinkimbi yokuvelisa i-penicillin.Zombini i-1,3-DAP kunye ne-spermidine, kodwa i-purescine (i-1,4-diaminobutane), ivelisa ukwanda okukhulu kumanqanaba e-transcript ye-penicillin biosynthetic genes pcbAB, pcbC kunye ne-penDE.Ezi polyamines azichaphazeli ukubonakaliswa kwe-pH-stress regulator pacC gene yehlabathi, ngaloo ndlela ingabandakanyi ukuba umphumo we-1,3-DAP kunye ne-spermidine ngenxa yokuguqulwa kwendlela yokulawula i-pH.Ukubonakaliswa kweentlobo ezintathu ze-penicillin biosynthetic gene zehla kakhulu kwi-laeA-knock-down mutant ye-P. chrysogenum, evelisa amanqanaba aphantsi kakhulu e-benzylpenicillin.Okubangela umdla kukuba, i-1,3-DAP kunye ne-spermidine zibuyisela impembelelo ye-laeA yokuguqa, ibuyisela ngokupheleleyo amanqanaba emveliso ye-penicillin.Ngaphaya koko, i-1,3-DAP kunye ne-spermidine zaphucula ukubonakaliswa kwe-laeA kuxinzelelo lwabazali kwaye zabuyisela amanqanaba e-laeA transcripts kwi-laeA knock-down mutant.Kuthathwe kunye ezi ziphumo zibonisa ukuba impembelelo yokuvuselela i-inducer molecules i-1,3-DAP kunye ne-spermidine iqhutywe, ubuncinane ngokuyinxalenye, ngokukhuthazwa kwentetho ye-laeA, umlawuli wehlabathi jikelele owenza i-epigenetically kwi-expression ye-second metabolite genes. ngohlengahlengiso lwe-heterochromatin.
