I-Spiramycin Cas: 8025-81-8
| Inombolo Yekhathalogu | XD90452 |
| Igama lemveliso | I-Spiramycin |
| CAS | 8025-81-8 |
| Ifomula yeemolekyuli | I-C43H74N2O14 |
| Ubunzima beMolekyuli | 843.05 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29419000 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo oMhlophe |
| Isivavanyi | >4100IU/mg |
| Iintsimbi ezinzima | < 20ppm |
| Ilahleko ekomisweni | < 3.5% |
| Uthuthu lweSulfate | < 1.0% |
| I-Ethanol | < 2.0% |
| Ukujikeleza okuthe ngqo | -85 ukuya -80 deg |
I-Streptomyces ambofaciens yenza i-macrolide antibiotic spiramycin.Iqela lemfuza ye-biosynthetic ye-spiramycin iphawulwe kwi-S. ambofaciens.Ukongeza kwi-gene yokulawula i-srmR (srm22), echongiweyo ngaphambili (M. Geistlich et al., Mol. Microbiol. 6: 2019-2029, 1992), iijini zokulawula ezibekayo ezintathu ziye zachongwa ngokuhlalutya ngokulandelelana.Uhlalutyo lwentetho yemfuza kunye nemifuniselo yokungasebenzisi yemfuza ibonise ukuba inye kuphela kwezi zintathu zofuzo, i-srm40, edlala indima enkulu kulawulo lwe-spiramycin biosynthesis.Ukuphazamiseka kwe-srm22 okanye i-srm40 kuphelise ukuveliswa kwe-spiramycin ngelixa ukubonakaliswa kwayo ngokugqithisileyo kunyuse imveliso ye-spiramycin.Uhlalutyo lwenkcazo lwenziwa ngokuguqulelwa kwe-reverse-PCR (RT-PCR) kuzo zonke iigenes zeqela kwi-wild-type strain kunye ne-srm22 (srmR) kunye ne-srm40 yokucima i-mutants.Iziphumo ezisuka kuhlahlelo lwentetho, kunye nezisuka kuvavanyo lokuzalisekisa, zibonise ukuba i-Srm22 iyafuneka kwintetho ye-srm40, i-Srm40 isisivuseleli sendlela ethile elawula uninzi, ukuba asilulo lonke, lwe-spiramycin biosynthetic gene.
