Iimveliso

I-Cysteine ​​​​(i-Cys) idlala indima ebalulekileyo kwiinkqubo ezininzi ze-physiological yeeseli ze-eukaryotic kwaye ukufunyanwa kwayo kwiiseli kubaluleke kakhulu.Nangona kunjalo, i-glutathione (GSH), i-homocysteine, i-N-acetyl-L-cysteine ​​kunye nezinye i-thiols zithintela kakhulu ukufunyanwa kweCys.Ngokukodwa, i-GSH iphazamisa kakhulu ukufunyanwa kwee-Cys zeselula (30-200 μM) ngenxa yoxinzelelo oluphezulu lwe-intracellular (1-10 mM).Kulo msebenzi, i-off-on fluorescent probe (HOTA) yokuchongwa kweCys ibonakalisiwe.Olu phononongo lunobuntununtunu obugqwesileyo kunye nokukhetha okwanelisayo ekubhaqweni kwe-Cys zeselula: kunye nokongezwa kwe-200 μM Cys, ukuqina kwe-fluorescence yeprobe (10 μM) eyomeleziweyo ka-117 kwaye umda wokubhaqa ubalwe ukuba yi-13.47 μM, engaphantsi. kunogxininiso lwe-Cys yeselula;i-probe iphinde yafumanisa ngokukhetha i-30-200 μM i-cysteine ​​​​ngaphezu kwe-1-10 mM glutathione.Ngenxa yoko, imifuniselo yemifanekiso yeeseli yenziwa ngeprobe HOTA.Ngaphezu koko, iziphumo ze-thiol-blocki ng kunye ne-GSH synthesis inhibiting experiments ziqinisekisile ukuba ukukhutshwa kwe-intracellular ikakhulu kuvela kwintsebenziswano phakathi kweCys kunye ne-HOTA.