X-GAL CAS:7240-90-6 98% Mhlophe ukuya kumhlophe-mhlophe Crystalline Powder
| Inombolo Yekhathalogu | XD90008 |
| Igama lemveliso | I-X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Ifomula yeemolekyuli | I-C14H15BrClNO6 |
| Ubunzima beMolekyuli | 408.63 |
| Iinkcukacha zokuGcina | -2 ukuya -6 °C |
| IKhowudi yoMrhumo eHarmonized | 29400000 |
Ukucaciswa kweMveliso
| Imbonakalo yesisombululo | Isisombululo esicacileyo, esingenambala ukuya kokukhanya tyheli (50mg/ml kwiDMF:MeOH, 1:1) |
| Ukujikeleza okuthe ngqo | -61.5 +/- 1 |
| Imbonakalo | Umgubo wekristale omhlophe ukuya kumhlophe |
| Ubunyulu HPLC | umz 99% |
| Ukunyibilika (5 % kwiDMF) | Inyibilika (5% w/v,DMF) |
| Amanzi KF | ubuninzi 1% |
| I-Assay (HPLC kwi-Anhydrous Basis) | umz 98% w/w |
Ukusetyenziswa kwe-X-gal
I-X-gal (iphinde ishunqulelwe i-BCIG ye-5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) i-organic compound equkethe i-galactose edityaniswe ne-indole efakwe endaweni.Ikhompawundi yadityaniswa nguJerome Horwitz kunye nabasebenzisana nabo ngo-1964.Igama lekhemikhali elisesikweni lihlala lishunqulelwe ukuba libe mfutshane kodwa libe namabinzana anzima kakhulu afana ne-bromochloroindoxyl galactoside.I-X evela kwi-indoxyl ingaba ngumthombo we-X kwi-contraction ye-X.I-X-gal isoloko isetyenziselwa i-biology ye-molecular ukuvavanya ubukho be-enzyme, i-β-galactosidase, kwindawo ekujoliswe kuyo eqhelekileyo, i-β-galactoside.Ikwasetyenziselwa ukubona umsebenzi wale enzyme kwi-histochemistry kunye ne-bacteriology.I-X-gal yenye yee-indoxyl glycosides ezininzi kunye nee-esters ezivelisa iikhompawundi eziluhlaza ezinganyibilikiyo ezifana nedayi ye-indigo njengesiphumo se-enzyme-catalyzed hydrolysis.
I-X-gal yi-analog ye-lactose, kwaye ngoko ke inokuthi ifakwe i-hydrolyzed yi-enzyme ye-β-galactosidase eqhekeza i-β-glycosidic bond kwi-D-lactose.I-X-gal, xa icolwe yi-β-galactosidase, ivelisa i-galactose kunye ne-5-bromo- 4-chloro-3-hydroxyindole - 1. Le yokugqibela ke ngokuzenzekelayo iyancipha kwaye ifakwe kwi-5,5'-dibromo-4,4'-dichloro -indigo - 2, imveliso eluhlaza kakhulu enganyibilikiyo.I-X-gal ngokwayo ayinambala, ngoko ke ubukho bemveliso enombala oluhlaza ke ngoko bunokusetyenziswa njengovavanyo lobukho be-β-galactosidase esebenzayo.Oku kukwavumela i-bacterial β-galactosidase (ebizwa ngokuba yi-lacZ) ukuba isetyenziswe njengentatheli kwizicelo ezahlukeneyo.
Kuhlalutyo lwama-hybridi amabini, i-β-galactosidase ingasetyenziselwa njengentatheli ukuchonga iiprotheni ezisebenzisanayo.Ngale ndlela, iilayibrari ze-genome zinokujongwa ukusebenzisana kweprotheyini kusetyenziswa igwele okanye inkqubo yebhaktheriya.Apho kukho ukusebenzisana okuyimpumelelo phakathi kweeprotheni ezihlolwe, kuya kubangela ukubophelela kwesizinda sokusebenza kumgqugquzeli.Ukuba umgqugquzeli udibaniswe ne-lacZ gene, ukuveliswa kwe-β-galactosidase, okubangela ukubunjwa kwamakholoni e-blue-pigmented phambi kwe-X-gal, ngoko kuya kubonisa ukusebenzisana okuphumelelayo phakathi kweeprotheni.Obu buchule busenokulinganiselwa ekuvavanyeni amathala eencwadi angaphantsi kobukhulu obungaphantsi kwe-106. Ukucandwa okuyimpumelelo kwe-X-gal kukwadala ivumba elibi elibonakalayo ngenxa yokuguquka kwe-indole.
Njengoko i-X-gal ngokwayo ingenambala, ubukho bemveliso enombala ozuba bunokusetyenziswa njengovavanyo lobukho be-β-galactosidase esebenzayo.
Oku kuchongwa lula kwe-enzyme esebenzayo kuvumela i-gene ye-βgalactosidase (i-lacZ gene) ukuba isetyenziswe njenge-reporter gene kwizicelo ezahlukeneyo.
