Iiprotheyini zeprosesa yesignali ye-PII zisasazeka ngokubanzi kwiiprokaryotes kunye nezityalo apho zilawula ubuninzi be-anabolic reactions.Ukuveliswa ngokugqithisileyo kwe-metabolites kufuna ukukhulula iisekethe zokulawula iiselula eziqinileyo.Apha sibonisa ukuba inqaku elinye lokuguqulwa kweprotheyini ye-PII evela kwi-cyanobacterium Synechocystis sp.I-PCC 6803 yanele ukuvula indlela ye-arginine ebangela ukuqokelelana kwe-biopolymer cyanophycin (i-multi-L-arginyl-poly-L-aspartate).Le mveliso inomdla we-biotechnological njengomthombo we-amino acids kunye ne-polyaspartic acid.Lo msebenzi ungumzekelo wendlela entsha yobunjineli bendlela ngokuyila iiproteni zokubonisa i-PII ezilungiselelwe ngokwesiko.Apha, i-Synechocystis sp eyenziwe ngobunjineli.I-PCC6803 yoxinzelelo kunye ne-PII-I86N yokuguqulwa kwe-arginine engaphezulu-iqokelelwe ngokusetyenziswa kwe-enzyme engundoqo i-N-acetylglutamate kinase (NAGK). kunokuba kuhlobo lwasendle.Ngenxa yoko, uhlobo lwe-BW86 luqokelele ukuya kuthi ga kwi-57% ye-cyanophycin ngeseli yobunzima bomileyo phantsi kweemeko ezivavanyiweyo, esona sivuno siphezulu se-cyanophycin esixeliweyo ukuza kuthi ga ngoku.Uxinzelelo lwe-BW86 luvelise i-cyanophycin kuluhlu lwe-molecular mass 25 ukuya>>100 kDa;uhlobo lwasendle luvelise i-polymer kuluhlu lwe-30 ukuya>> 100 kDa. Isivuno esiphezulu kunye nobunzima be-molecular mass ye-cyanophycin eveliswe ngoxinzelelo lwe-BW86 kunye neemfuno eziphantsi zezondlo ze-cyanobacteria zenza ukuba kube yindlela ethembisayo yokuveliswa kwe-biotechnological ye-cyanophycin.Olu phononongo lukwabonisa ukuba nokwenzeka kobunjineli bendlela yemetabolism kusetyenziswa iprotein ye-PII yokubonisa, eyenzeka kwiibhaktiriya ezininzi.
