Iimveliso

I-aptasensor esebenzayo yaphuhliswa apho i-graphene oxide (GO) yaqeshwa njengesalathisi kuzo zombini i-electrochemical impedance spectroscopy kunye ne-electrochemiluminescence (ECL) yokuvelisa uphawu.I-aptasensor yenziwe ngokuzihlanganisela ngokwazo i-ECL probe ye-thiolated adenosine triphosphate binding aptamer (ABA) ephawulwe ngeRu complex (Ru(bpy)3(2+) derivatives) kumphezulu we nanoparticle yegolide (AuNP) eguqulweyo yeglass carbon i-electrode (GCE).I-ABA ingashukumi kwi-AuNP elungisiweyo ye-GCE inokuthengisa ngamandla i-GO ngenxa yobudlelwane olomeleleyo π-π phakathi kwe-ABA kunye negraphene oxide;Ukucima i-ECL ye-Ru complex emva koko kwenzeka ngenxa yokudluliselwa kwamandla kunye nokudluliselwa kwe-electron, kunye nokunyuka okukhulu kwe-electron transfer resistance (Ret) ye-electrode.Ngelixa kubukho bethagethi ye-adenosine triphosphate (ATP), i-ABA ikhetha ukwenza i-ABA-ATP bioaffinity complexes, enobudlelwane obubuthathaka kwi-graphene oxide kwaye igcine i-graphene oxide kude nomphezulu we-electrode, ngaloo ndlela ivumela ukuphuculwa komqondiso we-ECL, kunye ngokubambisana nokuncipha kweRet.Ngenxa ye-ECL ephezulu yokucima ukusebenza kakuhle, isakhiwo esiyingqayizivele, kunye neepropati ze-elektroniki ze-graphene oxide, i-Ret kunye ne-ECL intensity ngokubhekiselele kwi-logarithm ye-ATP yoxinaniso yayinomgca kuluhlu olubanzi ukusuka kwi-10 pM ukuya kwi-10 nM kunye nomda wokufumanisa i-ultra-low ye-6.7 pM ukuya ku-4.8 pM, ngokulandelelanayo.I-aptasensor ecetywayo ibonise ukuveliswa kwakhona okugqwesileyo, ukuzinza, kunye nokukhetha okubalaseleyo, kwaye i-ATP inokwahlulwa ngokufanelekileyo kwii-analogues zayo.Okubaluleke ngakumbi, esi sicwangciso sisebenzayo se-ECL se-aptasensor esekwe kwi-GO isebenza zombini njenge-electrochemical kunye nesalathisi somqondiso we-ECL sibanzi kwaye sinokwandiswa ngokulula kwezinye iziganeko ezibophelelayo zebhayoloji.