I-isomeric bicyclo [4.1.0] i-heptane analogues ye-glycosidase inhibitor galacto-validamine, (1R *, 2S, 3S, 4S, 5S, 6S *) -5-amino-1- (hydroxymethyl) bicyclo [4.1.0]heptane -2,3,4-triol, iye yaqulunqwa ngamanyathelo angama-13 ukusuka kwi-2,3,4,6-tetra-O-benzyl-D-galactose.Imisebenzi yokuthintela yee-amines ezimbini ezithintelweyo, kunye nee-acetamide ezihambelana nazo, zilinganiswe ngokuchasene ne-alpha-galactosidase enzymes zorhwebo ezivela kwimbotyi yekofu kunye ne-E. coli.Umsebenzi we-glycosyl hydrolase intsapho ye-GH27 enzyme (ibhontshisi yekhofi) yayinqatshelwe ngokukhuphisana ne-1R, 6S-amine (7), intsebenziswano ebophelelayo ebonakaliswe yi-K (i) ixabiso le-0.541 microM.I-GH36 E. coli alpha-galactosidase ibonise intsebenziswano ebophelelayo ebuthakathaka kakhulu kunye ne-1R, 6S-amine (IC (50) = 80 microM).I-diastereomeric 1S, i-6R-amine (9) ibophelelwe buthathaka kuzo zombini i-galactosidases, (ibhontshisi yekhofi, i-IC (50) = 286 microM) kunye (E. coli, IC (50) = 2.46 mM).
