![3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98% Umfanekiso ofakiweyo](http://cdn.globalso.com/xdbiochems/75621-03-3.jpg)
3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
| Inombolo Yekhathalogu | XD90075 |
| Igama lemveliso | 3 - [(3-Cholanidopropyl) dimethylammonio] -1-propanesulfonate |
| CAS | 75621-03-3 |
| Ifomula yeemolekyuli | I-C32H58N2O7S |
| Ubunzima beMolekyuli | 614.88 |
| Iinkcukacha zokuGcina | I-Ambient |
| Ingqinelanisiwe IKhowudi yoMrhumo | 29242970 |
Ukucaciswa kweMveliso
| IR | Ukuthelekiswa nereferensi |
| Ilahleko ekomisweni | <2% |
| TLC | Indawo enye |
| Nitrogen | 4.46 - 4.65 |
| Imbonakalo | Umgubo omhlophe |
| Ukunyibilika (10 % kwi-H2O) | Icacile kwaye ayinambala |
| Ukuqhuba (0.1M kwi-H2O) | <10us/cm |
| Uvavanyo lwe-HPLC | >98% |
| UV (5% kwi-H2O) A290nm | <0.5 |
| UV (5% kwi-H2O) A400nm | <0.02 |
| I-Solvent Content (nge-GC) i-Dimethylformamide | <2.0% |
3--[3-(cholamidopropyl) dimethylamino] propanesulfonic acid ukusetyenziswa kwetyuwa yangaphakathi kunye nendlela yokudibanisa
IBiological Activity: CHAPS, iphuma kwiCholicacid, sisicoci sezwitterionic esinyibilikisa iiproteni zenwebu.I-CHAPS isetyenziselwa ukuzinzisa iiprotheyini ezahlukeneyo ze-DNA kwaye inokugcina umsebenzi we-biochemical of proteins kwisisombululo.
Usetyenziso: I-CHAPS sisicoci esinga-denaturing se-zwitterionic se-membrane biochemistry.Ingasetyenziselwa ukunyibilikisa iiproteni ze-membrane kunye nokuphazamisa ukusebenzisana kweprotheni-protein.I-CHAPS inobunzima obuphantsi be-micellar molecular (6,150) kunye noxinzelelo oluphezulu lwe-micelle (6-10 mM) kwaye ngoko inokususwa kwisampuli nge-dialysis.Ikwafanelekele ukunyibilikisa iiprotheni kwi-isoelectric focusing kunye ne-2D electrophoresis.I-CHAPS iqhele ukusetyenziswa kugxininiso lwendalo (i-urea-free) ye-isoelectric kwaye ifunyenwe inika isisombululo esigqwesileyo kumalungiselelo athile asezantsi kunye neeproteni zezityalo.Ngokuqhelekileyo ugxininiso phakathi kwe-2-4% (w / v) isetyenziselwa i-isoelectric focusing gels.
Ukusetyenziswa: Isicoci se-zwitterionic esingena-denaturing se-membrane biochemistry, esinokusetyenziselwa ukunyibilikisa iiproteni ze-membrane kunye nokuphazamisa ukusebenzisana kweprotheni-protein;kwakhona ilungele ukunyibilikisa iiprotheni kwi-isoelectric focusing and two-dimensional electrophoresis;isetyenziswa ngokuqhelekileyo kwi-Non-denaturing (akukho urea) igxile kwi-isoelectric;I-RNA kunye ne-DNA extraction, akukho msebenzi we-RNase kunye ne-DNase
Usetyenziso: I-Amphoteric surfactant, i-CHAPS sisicoci se-zwitterionic esinga-denaturing, i-protein lysate yokunyibilikisa iiproteni ze-membrane kunye nokudibana kwe-lysing phakathi kweeprotheni kunye neeprotheni.
