Iimveliso

Intshayelelo:I-Tris-Hydroxymethylaminomethane hydrochloride ibizwa ngokuba yi-Tris-HCl.I-Tris-HCl buffer (i-Tris-HCl buffer) ayisetyenziswanga ngokubanzi njenge-solvent ye-nucleic acids kunye neeprotheni, kodwa inezinto ezininzi ezibalulekileyo.

Isicelo:I-Tris-HCl yayisetyenziselwa ukukhula kwekristale yeprotheni kwiimeko ezahlukeneyo ze-pH.Amandla e-ionic aphantsi e-Tris-HCL buffer angasetyenziselwa ukuqulunqwa kweentsinga eziphakathi kwe-lamin kwi-C. elegans.I-Tris-HCl ikwangomnye wamacandelo aphambili e-protein electrophoresis buffers.Ukongeza, i-Tris-HCL ikwayindawo ephakathi ukulungiselela i-surfactants, i-vulcanization accelerators kunye namanye amachiza e-Chemicalbook.I-Tris-HCl nayo yasetyenziswa njengomgangatho wetitration.

Ukulungiswa kwesisombululo sesithinteli:thatha i-tris(i-hydroxymethyl) i-aminomethane hydrochloride njenge-solute, thatha amanzi adibeneyo njenge-solvent, kwaye usebenzise isisombululo se-HCl samanzi kunye noxinzelelo lwe-0.1mol / L ukulungisa ixabiso le-pH ukulungiselela isisombululo se-trimethylolamine kunye noxinzelelo lwe-6.057g / L kunye ne-pH=8.8.Isisombululo se-Hydroxymethylaminomethane hydrochloride buffer (i-Tris-HCl buffer).

Iinkcazelo ngeMichiza:Iikristale ezimhlophe.

Ukusetyenziswa:I-pH yazo zonke izithinteli bubushushu kunye noxinzelelo.Kwi-Tris buffer, i-pH yonyuka malunga neeyunithi ze-0.03 nge-1 °C yokwanda kweqondo lobushushu kwaye yehla nge-0.03-0.05 yeeyunithi ngokuphinda-phindwe kayi-10 kokuhlanjululwa.

Isicelo:kwisithinteli sebhayoloji

Usebenzisa:isetyenziswe njengeziphakathi kwi-organic synthesis