Iimveliso

I-Chemifluorescence: I-molecular luminol yi-molecule ye-fluorescent yekhemikhali enokuthi iguqulwe ibe yi-aminophthalic acid yesimo esichulumancisayo phambi kweemolekyuli ze-hydrogen peroxide, ezikhupha i-fluorescence enamandla.I-hydrogen peroxide yimveliso yeempendulo ezininzi ze-biooxidative, ngoko kulula ukudibanisa ezi mpendulo ze-biooxidative kunye ne-photodetection ngokwazisa i-luminol.Ngokomzekelo, i-glucose oxidase / i-catalase probe inokubona ukuxinwa kwe-hydrogen peroxide okanye i-glucose kwisampuli, kwaye ixesha lokuphendula li-0.5 kuphela (indlela eguquguqukayo).Ukudibanisa izinto ze-chemiluminescent kunye ne-immunological reactions, kunye nokuvakalisa ukuxinwa kwamalungu omzimba afunyenweyo ngokuphendula ukukhanya, kubizwa ngokuba yi-chemiluminescent immunoassay technology.Kwi-1976, u-Shmeder ucebise i-chemiluminescence immunoassay, usebenzisa i-luminol-H202 kunye ne-ABEI ephuma kuyo njengenkqubo yokubonisa ukusabela.Okwangoku, i-luminol kunye nezinto eziphuma kuyo zezona ziphawulwayo zisetyenziswa kakhulu kwi-chemiluminescence immunoassay applications.Ngaphantsi kweemeko ze-alkaline, i-catalyzed yi-microperoxidase, inani elikhulu leefotoni lingakhutshwa.I-Luminol ingasetyenziselwa i-WesternBlot ye-HRP-ilebhile i-antibodies kunye ne-nucleic acid hybridization yokufumanisa iiprobe ezibhalwe nge-HRP.Ikwasetyenziswa ekubhaqweni kwe-bloodstain yabacuphi bale mihla.I-compound enokuthi isetyenziswe njenge-marker chemiluminescent kufuneka ihlangabezane nezi meko zilandelayo: isivuno se-quantum se-luminescence siphezulu;iimpawu zayo ze-physicochemical zihambelana nenkqubo ephantsi kwesifundo;ukusabela kwayo kwe-luminescent yisiphumo sokusabela kwe-oxidation ye-luminescent substance;ngaphakathi koluhlu loxinaniso olusetyenziswa Ngaphakathi ezingenayo ityhefu kwizinto eziphilayo.Iindidi ezininzi ze-chemiluminescence reagents zisetyenziswa ngokuqhelekileyo: i-acridine ester, i-chemiluminescence tracer esetyenziswa ngokubanzi, i-tricyclic organic compound, i-oxidize elula, kunye ne-oxidation reaction ayifuni i-catalyst, kwaye ikhupha i-photons kwi-430nm;I-Luminol kunye ne-isoluminol kunye ne-derivatives yazo zezona zivuthiweyo ze-chemiluminescent agents.Kwangowe-1964, Ngelixa ingxelo malunga ne-chemiluminescence, i-luminol, i-isoluminol kunye nezinto eziphuma kuzo zazisetyenziselwa i-chemiluminescence.I-Immunoassays iphumelele iziphumo ezingcono.Ukubonwa kweLuminescence: obona bude bungu-fluorescence waveleng yi-400nm (ukubonwa kwe-chemiluminescence kwi-60mmK2S2O8, 100mmK2CO3, isisombululo se-pH11.5)

I-Chemiluminescence: Njengendlela yokuvavanya ebucayi, i-chemiluminescence isetyenziswa ngokubanzi ukufumana i-radicals yamahhala kunye ne-reaction metabolites eveliswa kwii-enzymes, iiseli kunye nezinto eziphilayo.Ukukhanya okuphuma kwiimveliso zabo ze-oxidation kunye ne-metabolites kunokusetyenziswa ngeefotometer ezahlukeneyo.ukubhaqwa.I-Chemiluminescence isetyenziswa ngokubanzi kwi-screening kunye nophando lwe-antioxidants, njenge-polyphenols, i-polysaccharides, i-flavonoids, kunye ne-anthraquinones, ngenxa yobuntu bayo, ukukhawuleza, ukusebenza okulula kunye nexabiso eliphantsi.Iinkqubo ze-chemiluminescence ezisetyenziswa ngokuqhelekileyo zokumisela i-anions ye-superoxide ziquka i-xanthine oxidase-luminol, i-pyrogallol-luminol kunye ne-dimethyl sulfoxide-luminol.Eyokuqala yinkqubo ye-enzymatic, kwaye ezimbini zokugqibela Yinkqubo engeyiyo i-enzymatic.Iinkqubo ze-chemiluminescence zokumisela i-hydroxyl radicals ikakhulu ziquka i-copper sulfate-yeast (okanye iiseli zomongo wamathambo) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen Iinkqubo zeperoxide 5 chemiluminescence, i-ferrous sulfate-luminol-hydrogen peroxide kunye ne-ferrous sulfate-luminol, zonke zibandakanya impendulo ye-Fenton yakudala ukuvelisa i-hydroxyl radicals, kwaye emva koko ihlasele i-agent ye-luminescent ukuvelisa i-chemiluminescence, enokusetyenziswa ukulinganisa ukususwa kwezicatshulwa. umsebenzi we-oxygen radicals esebenzayo.Indlela yokuvavanya: sebenzisa inkqubo ye-pyrogallol-luminol chemiluminescence: i-luminol yenziwe kwisisombululo se-0.05mol / L ye-concentration kunye ne-0.05mol / L isisombululo se-NaOH, egcinwe kwindawo emnyama, kunye namanzi aphindwe kabini ngaphambi kokusetyenziswa Dilute kwi-1mmol / L isisombululo, sebenzisa i-1mmol / L HCl ukwenza isisombululo se-0.01mol / L se-pyrogallol, sigcine efrijini kwi-4 ° C, kwaye uhlambulule ngamanzi adibeneyo aphindwe kabini ukuya kuma-16 amaxesha angama-6.25 × 10-4mol / L ngaphambi kokusetyenziswa.I-0.05mol / L pH10.2Na2CO3-NaHCO3 i-buffer (equkethe i-0.1mmol / LEDTA) yalungiswa ngaphambi kokusetyenziswa, kwaye ixutywe ne-1mmol / L luminol kwi-2: 1 (iqhekeza levolumu) ukwenza i-luminol kunye ne-carbonate buffer phambi komxube wokulinga.Ngexesha lokulinganisa, tofa i-10.0 μL iisampulu zoxinaniso olwahlukileyo (0, 0.08, 0.4, 2 kunye ne-10 mg/mL) kwiseli ekhanyayo (ngesampulu yesithinteli njengolawulo), uze utofe i-6.25×10-4mol/L pyrogallol. 0.05 Ekugqibeleni, i-0.94 mL yomxube we-luminol kunye ne-carbonic acid buffer yongezwa ukuqalisa ukusabela (30 ° C), ukunyanzeliswa kwe-luminescence kubalwa ngexesha le-2 s, kwaye i-luminescence intensity edibeneyo edibeneyo kwi-300 ilinganiswe.Imvelaphi ye-luminescence intensity yayiyi-luminescence xa i-pyrogallol yayingafakwanga.ixabiso.Ukongeza, kukho i-chemiluminescence, i-fluorescence spectroscopy kunye ne-NBS-dichlorofluorescein chemiluminescence inkqubo yokumisela i-fluorescence chemiluminescence, njl., inzuzo yabo enkulu yimvakalelo ephezulu.

Iimpawu zeMichiza: Umgubo wekristale otyheli.Inyibilika ngokulula kwilye, inyibilika kwiasidi enyibilikayo, phantse ayinyibiliki emanzini, kwaye ayinyibiliki etywaleni.Izisombululo ezingathathi hlangothi okanye ezineasidi kancinane zibonisa i-fluorescence eqaqambileyo eluhlaza xa ibekwe kukukhanya kwe-ultraviolet.Indawo yokunyibilika 329-332℃

Ukusetyenziswa: Njenge-reagent yohlalutyo lweekhemikhali kunye nesalathisi.Sebenzisa uhlalutyo lwe-Chemiluminescence yokufumanisa i-reagent (efana nokumiselwa kweecations zetsimbi okanye igazi)

Ukusetyenziswa: Uhlalutyo lwe-chemiluminescence, olunje: i-cations yesinyithi, igazi kunye ne-glucocorticoids

Ukusetyenziswa: Uvavanyo lwe-Luminescence: i-Emmax440nm (i-chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; emva kokongeza i-H2O2) i-chemiluminescence reagent kunye nesalathisi, esisetyenziswa ngokuqhelekileyo kuhlalutyo lwe-chemiluminescence, njenge-cations zetsimbi, ukugonywa kwegazi, njl.