I-TMB Cas: 54827-17-7 99% Emhlophe, imhlophe ukuya kugrey okanye umgubo omthubi
| Inombolo Yekhathalogu | XD90163 |
| Igama lemveliso | TMB |
| CAS | 54827-17-7 |
| Ifomula yeemolekyuli | I-C16H20N2 |
| Ubunzima beMolekyuli | 240.34 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29215990 |
Ukucaciswa kweMveliso
| Imbonakalo | Mhlophe, ukusuka mhlophe ukuya ngwevu okanye umgubo omthubi |
| uAsay | 99% |
| Ilahleko ekomisweni | <2.0% |
| Isetyenziselwa uphando kuphela, hayi ukusetyenziswa komntu | uphando lusetyenziselwa kuphela, kungekhona ukusetyenziswa komntu |
Iipropati: 3,3',5,5'-Tetramethylbenzidine yikristale emhlophe engumgubo, ayinavumba, ayinancasa, ayinyibiliki emanzini, inyibilika ngokulula kwi-acetone, i-ether, i-dimethyl sulfoxide, i-dimethylformamide, njl. Izinyibilikisi ze-Organic.
Ukulungiselela: I-3,3,5,5-Tetramethylbenzidine yinto ebalulekileyo ye-chromogen reagent.Ukusebenzisa i-2,6-dimethylaniline njengento eluhlaza, ngokusebenzisa i-activation, i-oxidative coupling kunye nokucoca, i-3,3,5,5-tetramethylbenzidine ecocekileyo ifunyenwe, kwaye isivuno esipheleleyo sifikelela kwi-65%.
Umsebenzi webhayoloji: i-TMB (BMblue) yi-chromogenic substrate ye-immunohistochemistry kunye ne-ELISA.
Ukusetyenziswa: Le mveliso i-non-carcinogenic endaweni ye-benzidine 1 (i-Ames test negative), ifanelekile njenge-peroxidase substrate ye-enzyme-linked immunosorbent assay.I-substrate ivelisa imveliso e-soluble bluish yokugqibela enokufundwa nge-spectrophotometrically kwi-370 okanye i-620-650 nm.I-reaction ye-TMB inokumiswa nge-2MH2SO4 (ijika iphuzi) kwaye ifundwe kwi-50 nm kwi-4Chemicalbook.I-reagent enovakalelo kunye nekhethekileyo yokukhangela igazi, ukulinganisa i-hemoglobin, kunye ne-peroxidase yokulinganisa.
Ukusetyenziswa: I-reagent entsha nekhuselekileyo yechromogen;I-TMB ngokuthe ngcembe ithathe indawo ye-benzidine eyomeleleyo ye-carcinogen kunye nezinye izinto eziphuma kwi-carcinogenic benzidine, kwaye isetyenziswa kuvavanyo lwezonyango, uvavanyo lwasenkundleni, ukubhaqwa kolwaphulo-mthetho kunye nokubeka iliso kokusingqongileyo kunye neminye imimandla;ngakumbi kwiimvavanyo zeklinikhi ye-biochemical, i-TMB, njenge-substrate entsha ye-peroxidase, isetyenziswe ngokubanzi kwi-enzyme immunoassay (EIA) kunye ne-enzyme-linked immunosorbent assay (ELISA);isetyenziswa ikakhulu kwezi projekthi zilandelayo: ukubonwa kwegazi lobugqi;Ukufunyanwa ngokukhawuleza kotywala ematheni;ukulungiswa kwemichilo yokuhlola umchamo;ukufunyanwa kwentsholongwane ye-hepatitis;uvavanyo lokufumanisa ukukhulelwa;ukuzimisela ngokukhawuleza kwe-glucose, i-hemoglobin, i-albumin egazini kunye nomchamo;uvavanyo lwegazi lobugqi;ukuzimisela kwexabiso le-granulocyte egazini, i-steroids, Ukufunyanwa kwehomoni zesini;ukuzimisela komsebenzi we-enzymatic;ukufumanisa ii-antigens, amajoni omzimba kunye nemathiriyeli yemfuzo
