4-Amino-3-hydrazino-1,2,4-triazol-5-thiol Cas: 28836-03-5 99% Umthubi ukuya kuluhlaza oqinileyo
| Inombolo Yekhathalogu | XD90146 |
| Igama lemveliso | 4-Amino-3-hydrazino-1,2,4-triazol-5-thiol |
| CAS | 28836-03-5 |
| Ifomula yeemolekyuli | C16H13NO3S·NH3 |
| Ubunzima beMolekyuli | 316.37 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 2923900090 |
Ukucaciswa kweMveliso
| Imbonakalo | Umthubi ukuya koluhlaza okuqinileyo |
| uAsay | ≥ 99% |
| Indawo yokunyibilika | 237°C(dec.)(lit.) |
| Ukunyibilika | NAOH: Inyibilika1 N |
| Amanzi anyibilikayo | Inyibilika emanzini, 1N NaOH, kunye nemethanol. |
1.Sifunde iimpembelelo ezithintelayo ze-isorhamnetin kwi-mushroom tyrosinase nge-inhibition kinetics kunye ne-computational simulation.I-Isorhamnetin inhibited reversibly inhibited tyrosinase ngendlela exubeneyo e-Ki=0.235±0.013 mM.Imilinganiselo ye-intrinsic kunye ne-1-anilinonaphthalene-8-sulfonate (ANS) -i-fluorescence ebophayo ibonise ukuba i-isorhamnetin ayizange ibangele utshintsho olubalulekileyo kwisakhiwo se-tertiary ye-tyrosinase.Ukufumana ingqiqo kwinkqubo yokungasebenzi, i-kinetics ibalwe kusetyenziswa imilinganiselo yekhefu lexesha kunye neempendulo ze-substrate eziqhubekayo.Iziphumo zibonise ukuba ukungasebenzi okubangelwa yi-isorhamnetin kwakuyindlela yokuqala yokusabela kunye neenkqubo ze-biphasic.Ukufumana ukuqonda okungakumbi, siye salinganisa i-docking phakathi kwe-tyrosinase kunye ne-isorhamnetin.Ukulinganisa kwaphumelela (amandla okubopha kwi-Dock6.3: -32.58 kcal / mol, kwi-AutoDock4.2: -5.66 kcal / mol, kunye noFred2.2: -48.86 kcal / mol), ebonisa ukuba i-isorhamnetin isebenzisana neentsalela ezininzi, ezifana njenge-HIS244 kunye ne-MET280.Esi sicwangciso sentsebenziswano ye-predic ting tyrosinase ngokudityaniswa ne-kinetics esekwe kwikhompawundi ye-flavanone inokungqina ukuba luncedo ekuhloleni izinto ezinokubakho zendalo ze-tyrosinase inhibitors.
2.I-Acid unfolding pathway ye-conalbumin (CA), i-monomeric glycoprotein esuka kumhlophe weqanda lesikhukukazi, iphandwe kusetyenziswa i-CD spectroscopy ekude nakufuphi ne-UV, i-intrinsic fluorescence emission, i-extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (Anilino-8-napthalene sulfonate) kunye nokusasazwa kokukhanya okuguquguqukayo (DLS).Siqwalasela utshintsho oluxhomekeke kwi-pH kulwakhiwo lwe-CA lwesibini nakwinqanaba lesithathu.Inomthonyama-njenge-α-helical isakhiwo sesibini kwi-pH 4.0 kodwa isakhiwo sokulahlekelwa kwi-pH 3.0.I-CA yayikhona kuphela njenge-pre-molten globule state kunye ne-molt globule state kwisisombululo kwi-pH 4.0 kunye ne-pH 3.0, ngokulandelanayo.Isiphumo se-pH kwi-conformation kunye ne-thermostability ye-CA ikhomba ukuxhathisa ubushushu kwi-pH engathathi hlangothi.Iziphumo ze-DLS zibonisa ukuba i-MG state yayikhona njengefom edibeneyo kwizisombululo ezinamanzi kunye ne-hydrodynamic radii ye-4.7 nm.Ukucinywa kwe-tryptophan fluorescence nge-acrylamide kuqinisekisa ngakumbi ukuqokelelana kwemeko ephakathi, ngokuyinxenye etyhiliweyo, phakathi kwamazwe asekuhlaleni kunye namazwe angatyhilekanga.
![N-[[bis[4-(dimethylamino)phenyl]amino]carbonyl] glycine isodiyam ityuwa Emhlophe ukuya kumgubo ongwevu-luhlaza oyikristale](http://cdn.globalso.com/xdbiochems/115871-19-7.jpg)
