Iibhaktheriya ze-Colonic, ezibonakaliswe yi-Bacteroides thetaiotaomicron, zidlala indima ebalulekileyo ekugcineni impilo yabantu ngokudibanisa iintsapho ezinkulu ze-glycoside hydrolases (GHs) ukuxhaphaza i-polysaccharides yokutya kunye nokusingatha i-glycans njengezondlo.Ukwandiswa kwentsapho ye-GH enjalo kubonakaliswa yintsapho ye-23 ye-GH92 glycosidases encoded yi-B. thetaiotaomicron genome.Apha sibonisa ukuba ezi zi-alpha-mannosidases ezisebenza ngendlela enye yokufuduswa ukuze kusetyenziswe i-N-glycans ebambayo.Isakhiwo sesithathu-dimensional se-GH92 mannosidases sichaza intsapho yeeprotheni ze-domain ezimbini apho i-catalytic centre ifumaneka kwi-interface yesizinda, ukubonelela nge-asidi (glutamate) kunye nesiseko (aspartate) uncedo kwi-hydrolysis kwi-Ca (2+) - indlela exhomekeke.Izakhiwo ezine-dimensional ezintathu ze-GH92 eziyinkimbinkimbi kunye ne-inhibitors zibonelela ngengqiqo kwi-speciality, indlela kunye nohambo oluhambelanayo lwe-catalysis.I-Ca(2+) idlala indima ebalulekileyo ekuncedeni ukugqwetha i-mannoside kude ne-ground-state yayo (4)C(1) i-chair conformation ngokubhekiselele kwimeko yotshintsho.
