Inoveli ye-β-glucosidase gene, bgl1G5, yenziwe yavela kwiPhialophora sp.I-G5 kwaye ibonakaliswe ngempumelelo kwi-Pichia pastoris.Uhlalutyo lolandelelwano lubonise ukuba i-gene iqulethe i-1,431-bp evulekileyo yokufundwa kwesakhelo esifaka iprotheni ye-476 amino acids.Ukulandelelana kwe-amino acid efunyenweyo ye-bgl1G5 ibonise ubunikazi obuphezulu be-85% kunye ne-β-glucosidase ephawulekayo evela ku-Humicola grisea ye-glycoside hydrolase intsapho 1. Xa kuthelekiswa namanye ama-fungal amaqabane, i-Bgl1G5 ibonise umsebenzi ofanayo ophezulu kwi-pH 6.0 kunye ne-50 ° C kunye ne-50 ° C. kwi-pH 5.0-9.0.Ngaphezu koko, i-Bgl1G5 ibonise ukufudumeza okulungileyo kwi-50 ° C (i-6 h isiqingatha sobomi) kunye nomsebenzi ophezulu ophezulu (54.9 U mg-1).Amaxabiso e-K m kunye ne-V max ukuya kwi-p-nitrophenyl β-D-glucopyranoside (pNPG) yayiyi-0.33 mM kunye ne-103.1 μmol min-1 mg-1, ngokulandelanayo.Uvavanyo oluthile lwe-substrate lubonise ukuba i-Bgl1G5 yayisebenza kakhulu kwi-pNPG, ibuthathaka kwi-p-nitrophenyl β-D-cellobioside (pNPC) kunye ne-p-nitrophenyl-β-D-galactopyranoside (ONPG), kwaye yayingenamsebenzi kwi-cellobiose.
