I-Biosynthesis ye-proteoglycans kunye ne-glycosaminoglycans kubukho be-p-nitrophenyl-xyloside yaphononongwa kusetyenziswa inkqubo yenkcubeko yeempuku ze-ovarian granulosa cell.Ukongezwa kwe-p-nitrophenyl-xyloside kwi-cellculture medium kubangele malunga ne-700% yokwanda kwe- [35S] sulfate incorporation (ED50 kwi-0.03 mM) kwii-macromolecules, ezibandakanya i-chondroitin sulfate chain yamahhala eqalwe kwi-xyloside kunye ne-proteoglycans yasekhaya.Amatyathanga e-chondroitin sulfate asimahla aqaliswe kwi-xyloside ayephantse afihlwa phakathi.Ubungakanani bemolekyuli yamatyathanga e-chondroitin sulfate behlile ukusuka kwi-40,000 ukuya kwi-21,000 njengoko iyonke [35S] yokufakwa kwesulfate iye yaphuculwa, nto leyo ebonisa ukuba ukuphuculwa kwe-synthesis ye-chondroitin sulfate yaphazamisa indlela eqhelekileyo yokupheliswa kwekhonkco le-glycosaminoglycan.I-Biosynthesis ye-heparan sulfate proteoglycans yancitshiswa malunga ne-50%, mhlawumbi ngenxa yokhuphiswano kwinqanaba le-UDP-precursors yeswekile.[35S]I-Sulfate incorporation yavalwa ngokongezwa kwe-cycloheximide kunye nesiqingatha sokuqala sexesha malunga ne-2 hr phambi kobukho be-xyloside, ngelixa ukungabikho kwe-xyloside kwakumalunga ne-20 min.Umahluko usenokuba ubonisa ireyithi yenguquko ye-glycosaminoglycan synthesizing umthamo xa iyonke.Izinga lenguquko ye-glycosaminoglycan synthesizing umthamo obonwe kwiiseli ze-ovarian granulosa lalimfutshane kakhulu kunelo libonwa kwii-chondrocytes, libonisa ukongamela okunxulumene nomsebenzi we-proteoglycan biosynthetic kumsebenzi we-metabolic uwonke weeseli.
