I-4-Nitrophenyl phosphate disodium ityuwa 6-hydrate CAS:333338-18-4 emhlophe ukuya kumgubo omthubi ophaphathekileyo
| Inombolo Yekhathalogu | XD90511 |
| Igama lemveliso | 4-Nitrophenyl phosphate disodium ityuwa 6-hydrate |
| CAS | 333338-18-4 |
| Ifomula yeemolekyuli | I-C6H16NNa2O12P |
| Ubunzima beMolekyuli | 371.142 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29199000 |
Ukucaciswa kweMveliso
| Imbonakalo | mhlophe ukuya kumthubi |
| Isivavanyi | 99% |
Ngaphezulu kwe-70% ye-gliomas ye-low-grade gliomas ithwala i-heterozygous R132H ukuguqulwa kwe-gene coding ye-isocitrate dehydrogenase 1 (IDH1).Oku kunika i-enzyme ngekhono lenoveli lokuguqula i-α-ketoglutarate kwi-2-hydroxyglutarate, ekugqibeleni ikhokelela kwi-tumorigenesis.Umthombo omkhulu wemveliso ye-2-hydroxyglutarate yi-glutamine, leyo, kumhlaza, ikwangumthombo we-tricarboxylic acid cycle (TCA) anaplerosis.Omnye umthombo we-anaplerosis yi-pyruvate flux nge-pyruvate carboxylase (i-PC), eyindlela eqhelekileyo kwii-astrocytes eziqhelekileyo.Injongo yolu phononongo yayikukufumanisa ukuba i-PC isebenza njengomthombo we-TCA anaplerosis kwiiseli eziguquguqukayo ze-IDH1 apho i-glutamine isetyenziselwa ukuveliswa kwe-2-hydroxyglutarate.Ii-astrocytes eziqhelekileyo zomntu ezingenakufa zenziwe ukuba zibonise i-heterozygous mutant IDH1 okanye i-IDH1 yasendle yaphandwa.Ukuqukuqela kwepyruvate nge-PC nange-pyruvate dehydrogenase (PDH) kumiselwe ngokusebenzisa i-magnetic resonance spectroscopy ukuphonononga ukufakwa kwelebhile ye- [2-¹³C] ephuma kwi-glucose ephuma kwi-¹³ileyibhile ye-C-led glutamate kunye ne-glutamine.Uvavanyo lomsebenzi, i-RT-PCR kunye ne-Western blotting zisetyenziselwe ukuphonononga intetho kunye nomsebenzi wee-enzyme ezifanelekileyo.Idatha yeCancer Genome Atlas (TCGA) yahlaziywa ukuvavanya ukubonakaliswa kwee-enzymes kwiisampuli ze-glioma yabantu.Xa kuthelekiswa neeseli zohlobo lwasendle, iiseli ze-IDH1 eziguquguqukayo zandisa kakhulu i-fractional flux nge-PC.Oku kwadibaniswa nokunyuka okuphawulekayo komsebenzi wePC kunye nokubonakalisa.Ngokufanayo, umsebenzi wePDH wehla kakhulu, mhlawumbi ulungelelwaniswa ngokunyuka kakhulu kwe-PDH phosphorylation ye-PDH ye-PDH 3. Ngokuhambelana nokuqwalaselwa kwiiseli, uhlalutyo lwedatha ye-TCGA lubonise ukwanda okukhulu kwintetho ye-PC kwi-IDH-ebonisa iisampulu ze-glioma zabantu xa kuthelekiswa nezilwanyana zasendle- thayipha i-IDH.Iziphumo zethu zibonisa ukuba utshintsho kwi-PC kunye ne-PDH lunokuba yinxalenye ebalulekileyo yokulungelelaniswa kweselula kwi-IDH1 yokuguqulwa kwaye inokusebenza njengeenjongo zonyango ezinokubakho.
