Acridine orange, hemi ityuwa Cas: 10127-02-3 Mfutshane
Inombolo Yekhathalogu | XD90520 |
Igama lemveliso | I-Acridine i-orange, ityuwa ye-hemi |
CAS | 10127-02-3 |
Ifomula yeemolekyuli | C17H20ClN3·½ZnCl2 |
Ubunzima beMolekyuli | 369.96 |
Iinkcukacha zokuGcina | I-Ambient |
IKhowudi yoMrhumo eHarmonized | 32129000 |
Ukucaciswa kweMveliso
Imbonakalo | Ubunzulu butyheli/obomvu/ ngumgubo omdaka |
Isivavanyi | 99% |
Ilahleko ekomisweni | <10% |
Ukufunxa okuthe ngqo | Min 1200 |
Ubude bamaza obuninzi bokufunxa | 488.0 - 498.0 |
Umlinganiselo wokufunxa | 0.90 - 1.90 |
Inkqubo yokuhlola ukulungiswa kwe-cytological yomlomo wesibeleko ichazwa esebenzisa i-Leitz Texture Analyzer System (E. Leitz, Rockleigh, NJ) i-quantitative staining kunye ne-acridine orange, kunye nomgangatho we-fluorescence.Isixhobo siskena iiseli kwizilayidi zemakroskopu kwaye sibhaqe izinto ezizitolika njengenuclei ezinobunzulu obugqithileyo benyukliya obuluhlaza befluorescence (Iziphumo ezidlulileyo zisebenzisa imilinganiselo yezandla zibonise ukuba ii-nuclei eziqhelekileyo azivelisi inyukliya yefluorescence eluhlaza iyonke enkulu kunomgangatho othile wobunzulu obucacileyo).Izinto ezichongiweyo zichongwa ngokujongwa okubonakalayo.Iiseli (102,000) ezivela kwizigulane ezingama-65 (i-29 eqhelekileyo, i-36 engaqhelekanga) ziye zavavanywa.Kwisampulu nganye engaqhelekanga, ubuncinane iseli enye engaqhelekanga yabhaqwa.Ngaphezulu kwesiqingatha seesampuli, ezinye izinto ezintathu okanye ezimbalwa (umz. i-clumps ye-polymorphonuclear leukocytes) zifunyenwe.Ezi zahlulahlulwe ngokulula kwinuclei enye, kwaye zinokulahlwa ngumntu onoqeqesho oluncinane kwi-cytological .