Iimveliso

Ubungqina bamva nje buxhasa indima evelayo ye-β-nicotinamide adenine dinucleotide (β-NAD (+)) njengenoveli ye-neurotransmitter kunye ne-neuromodulator kwinkqubo ye-nervous ye-peripheral -β-NAD (+) ikhutshwe kwi-nerve-smooth muscle preparations kunye neeseli ze-adrenal chromaffin. ngendlela yophawu lwe-neurotransmitter.Okwangoku akukacaci ukuba oku kuyinyani na kwi-CNS.Sisebenzisa i-superfusion assay yegumbi elincinci kunye ne-high-sensitivity high-pressure high-pressure liquid chromatography techniques, sibonisa ukuba i-K (+) ephezulu yokuvuselela i-rat forebrain synaptosomes ikhupha ukuphuphuma kwe-β-NAD (+) , i-adenosine 5'-triphosphate, kunye ne-triphosphate yabo. i-metabolites adenosine 5'-diphosphate (ADP), i-adenosine 5'-monophosphate, i-adenosine, i-ADP-ribose (ADPR) kunye ne-cyclic ADPR.I-high-K (+) -ikhukuliswe ukuphuphuma kwe-β-NAD (+) inqanyulwe kukuqhekeka kwe-SNAP-25 kunye ne-botulinum neurotoxin A, ngokuthintela i-N-uhlobo lwe-voltage exhomekeke kwi-Ca (2+) iziteshi ezine-ω-conotoxin GVIA. , kunye nokuthintela iproton gradient ye-synaptic vesicles ene-bafilo mycin A1, icebisa ukuba i-β-NAD(+) inokuthi ikhutshwe nge-vesicle exocytosis.Uhlalutyo lwaseNtshona lubonisa ukuba i-CD38, iprotheni esebenzayo eyenza i-metabolizes β-NAD (+) , ikhona kwi-synaptosomal membranes kunye ne-cytosol.I-synaptosomes engaguqukiyo ithoba i-β-NAD(+) .I-1,N (6) -etheno-NAD, i-analog ye-fluorescent ye-β-NAD (+) , ithathwa yi-synaptosomes kwaye oku kuthathwa kunqatshelwe yi-β-NAD (+) eyiyo, kodwa kungekhona nge-connexin 43 inhibitor Gap 27. Kwi-cortical neurons izicelo zendawo ze-β-NAD (+) zibangela i-Ca (2+) ngokukhawuleza, mhlawumbi ngenxa yokungena kwe-extracellular Ca (2+) .Ngoko ke, i-synaptosomes yobuchopho begundane inokukhulula ngokusebenzayo, ithobe kwaye ithathe i-β-NAD (+) , kunye ne-β-NAD (+) inokuvuselela i-postsynaptic neurons, zonke iikhrayitheriya ezifunekayo ukuze into ithathwe njenge-neurotransmitter yomviwa kwingqondo.