I-reagent entsha ye-Trinder yinto ephuma emanzini e-aniline enyibilikayo kakhulu esetyenziswa ngokubanzi kwiimvavanyo zokuxilonga kunye novavanyo lwe-biochemical.Ineengenelo ezininzi ngaphezulu kweerejenti zechromogenic eziqhelekileyo ekumiselweni kombala womsebenzi wehydrogen peroxide.I-reagent entsha ye-Trinder izinzile ngokwaneleyo kuzo zombini Inokusetyenziswa kwisisombululo okanye kwindlela yokubona umbhobho wokuvavanya.Phambi kwe-hydrogen peroxide kunye ne-peroxidase, i-reagent entsha ye-Trinder'si ingasetyenziswa ngokudibanisa ne-4-aminoantipyrine (4-AA) okanye i-3 methyl Ngexesha lokudibanisa i-oxidative reaction ye-benzothiazole sulfonehydrazone (MBTH), i-purple ezinzile kakhulu okanye idayi eluhlaza okwesibhakabhaka. zenziwe.I-molar absorbance of the dyes edityaniswe ne-MBTH yi-1.5-2 amaxesha aphezulu ngaphezu kweedayi ezidityaniswe ne-4-AA;kunjalo, 4-AA Isisombululo uzinzile ngaphezu MBTH isisombululo.I-substrate i-enzymatic oxidized yi-oxidase yayo ukwenza i-hydrogen peroxide.Ukugxininiswa kwale peroxide ye-hydrogen ihambelana nokuxinwa kwe-substrate.Ngoko ke, ubungakanani be-substrate bunokugqitywa ngokuphuhliswa kombala we-oxidative coupling reaction.I-Glucose, utywala, i-acyl-CoA, kunye ne-cholesterol ingasetyenziselwa ukukhangela ezo ndawo zihamba kunye ne-reagents ye-Trinder yenoveli kunye ne-4-AA.Kukho ii-reagents ezintsha ze-Trinder ezili-10.Phakathi kwe-reagents entsha ye-Trinder, i-TOOS yeyona nto isetyenziswa kakhulu.Nangona kunjalo, kwi-substrate ethile, ukuvavanywa kweentlobo ezahlukeneyo zee-reagents ze-Trinder kuyafuneka ukuze kuphuhliswe eyona nkqubo yokubona.
