Iimveliso

I-APS-5 i-substrate ye-chemiluminescent esekelwe kwi-9,10-dihydroacridine, eyona nto isetyenziselwa ukufumanisa i-ELISA yeekhompawundi kunye ne-alkaline phosphatase AP conjugates;I-9,10-dihydroacridine kwisakhiwo se-APS-5 Isakhiwo se-hydroacridine sinokukhupha ukukhanya ngokuqhubekayo, ngokuzinzileyo nangempumelelo phantsi kwesenzo se-AP, kunye ne-APS-5 ifaneleka kakhulu kwi-phosphatase i-assay solution solution kunye ne-phosphatase enzyme-linked immunoassay.

Njengophando olukhanyayo, lusetyenziswa kuphononongo lweetshiphu zemfuza.Ukuphendula kubhalwe nge-acridan (9,10-dihydroacridine) njenge-substrate kunye ne-alkaline phosphatase, evelisa i-chemiluminescence ephezulu eqhubekayo.Inika uvakalelo oluphezulu kunye nokulula kokusetyenziswa kwe-alkaline phosphatase conjugation ngexesha lokufumanisa i-chemiluminescent.I-alkaline phosphatase ifunyenwe ngaphantsi kwe-10-19 mol, iphakamileyo ngokukhawuleza ukunciphisa ixesha lokubona kunye nokwandisa i-throughput, kunye ne-slope ye-curve calibration curve ihlelwe nge-logarithmic elingana ne-1.0.Ubuninzi okanye ngaphezulu kwe-enzyme ivelisa enye okanye ngaphezulu kwe-luminescence, i-luminescence eqhubekayo-ayifuni kakhulu ngexesha lokuvavanya.I-Luminescence intensity inokufundwa kwi-curve ye-calibration calibration eveliswayo nangaliphi na ixesha, kwaye iziphumo zokuhlalutya azivakalelwa kwiqondo lokushisa kwi-22 ° C - 35 ° C, ukunciphisa ukuchaneka okufunekayo ukulawula ukushisa.

inzuzo:

I-1 Uvakalelo oluphezulu - iimpawu zeprotheni ezingaphantsi kwe-5pg zingabonwa;

2 Ukukhanya okuphezulu okukhanyayo - incopho ekhanyayo inokufikelelwa ngexesha elifutshane;

3 I-luminescence eqhubekayo kwaye ezinzileyo - inkqubo yokukhanya kwayo ayichatshazelwa bubushushu ngaphakathi kwe-25-35 ℃, kwaye ayifuni ukulawula ukushisa.

Ukuthelekiswa kwesicelo se-luminescent substrates esetyenziswa ngokuqhelekileyo i-APS-5 (193884-53-6) kunye ne-AMPPD (122341-56-4)

Zombini i-APS-5 (193884-53-6) kunye ne-AMPPD (122341-56-4) zii-chemiluminescent substrates ze-alkaline phosphatase, kwaye zombini zibizwa ngokuba zii-reagents zokubona uvakalelo lwe-ultra-high, ke ezi zixhobo zimbini zisekuchongeni ukuba yeyiphi engcono kwaye yeyiphi eyona imbi?Indlela yokukhetha xa usebenzisa?

Ngakolunye uhlangothi, xa i-APS-5 isetyenziswe njenge-alkaline phosphatase yokufumanisa i-substrate, uvakalelo lokufumanisa lunokufikelela kwi-10-19 yoxinaniso lwe-molar, ixesha lokuphuhlisa umbala linokufikelela kwincopho ephezulu malunga nemizuzwana ye-10, kwaye i-fluorescence ihlala ixesha elide. ixesha.Kule miba, i-APS 5 inokuthiwa ineempawu ezigqwesileyo.Nangona kunjalo, i-APS-5 ilinganiselwe kubushushu besicelo, kwaye iqondo lobushushu elinikezela ngombala likhethwa ukuba libe ngaphantsi kwe-20'C.Ngakolunye uhlangothi, xa i-AMPPD isetyenziswa njenge-substrate yokufumanisa i-alkaline phosphatase, uvakalelo lokubona lukwabizwa ngokuba yi-ultra-high sensitivity, kodwa ayikwazi ukufikelela kwinqanaba le-APS 5. Ixesha lokuphuhlisa umbala lifikelela kwincopho ephezulu malunga nemizuzwana ye-30. , kunye nobude be-fluorescence ubude.I-AMPPD igqwesileyo kwezi zakhiwo zingasentla, kodwa ingaphantsi kwe-APS5, kodwa ubushushu bayo bokunikezela ngombala alikho ngqongqo njenge-APS5, kwaye ubushushu bokugcina be-2--8C bulula kune-APS-5.Isishwankathelo, nangona i-APS-5 idlula i-AMPPD ekufumaneni ukuchaneka, ilahlekelwa kukuzinza.Indlela yokuyikhetha kuxhomekeke kwiimfuno ezithile zokubona.

1. Ukuqaliswa kwe-APS-5 (193884-53-6):

Igama lesiNgesi: APS-5

Inombolo yeCAS: 193884-53-6

Ifomula yemolekyuli: C21H15ClNO4PS Na2

Ubunzima bemolekyuli: 489.819

Ukubonakala: umgubo oqinileyo omhlophe omhlophe

Umxholo: ≥98%

Iinkcukacha: 1g, 5g, 10g

Iimeko zokugcina: -20 ℃ ukulondolozwa

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) ityuwa ye-phosphate disodium, ebizwa ngokuba yi-APS-5, i-reagent ye-chemiluminescent kwaye isetyenziswe njenge-substrate ye-chemiluminescent.Inika uvakalelo oluphezulu kunye nokulula kokusetyenziswa kwe-alkaline phosphatase conjugation ngexesha lokufumanisa i-chemiluminescent.Ukuphendula kubhalwe nge-acridan (9,10-dihydroacridine) njenge-substrate kunye ne-alkaline phosphatase, evelisa i-chemiluminescence ephezulu eqhubekayo.I-APS-5 yi-reagent efanelekileyo yokumisela umsebenzi we-phosphatase ngokuvavanywa kwesisombululo kunye ne-phosphatase enzyme-linked immunoassays.Njenge-luminescent substrate, i-APS-5 inokuqonda okuphezulu kunye nexesha elifutshane lokuphendula.

2. Isingeniso esicacileyo se-AMPPD (122341-56-4)

Igama lesiNgesi: AMPPD

Inombolo yeCAS: 122341-56-4

Ifomula yemolekyuli: C18H23O7P

Ubunzima bemolekyuli: 382.344781

Ukubonakala: umgubo omhlophe

Ubunyulu: ≥97%

Iinkcukacha: 1g, 5g, 10g

Iimeko zokugcina: -20 ℃ ukulondolozwa

Izilumkiso: Kufuneka yome ukuphepha ukuchithwa rhoqo kunye ne-lyophilization