I-antibody ye-monoclonal (mAb 8281) ecacileyo kwi-terminal β-galactose (βGal) ye-glycosphingolipids (GSL) kunye ne-glycoproteins yaveliswa kwiimpuku ezigonywe nge-lipid extract kwiiseli ezintsha ze-acute lymphocytic leukemia (ALL).I-Immuno-thin layer chromatography (ITLC) kunye novavanyo lokhuphiswano kunye nemigangatho ecocekileyo ye-GSL engathathi hlangothi, ishukela ekhululekile, kunye ne-synthetic neoglycoproteins ibonise i-mAb 8281 ukuba isebenze ngamandla kunye ne-LacCer, i-GalCer kunye ne-Gal-β-O- (CH3) 2S (CH3)2- I-CONH-(Gal-β-O-CETE) edityaniswe ne-albumin ye-bovine serum (BSA).Iswekile engaphambili nayo yadlala indima ekubopheni.I-antibody ayizange isebenze kunye ne-carbohydrates ene-terminal αGal izakhiwo kunye ne-terminal moieties ezinganxulumananga.Ukungcola okungathanga ngqo kwe-immunoperoxidase kunye nokuhamba kwe-cytometry kunye ne-mAb 8281 ibonise ibala elilungileyo kwiithishu ezininzi, kubandakanya imisipha egudileyo, i-gastrointestinal mucosa, i-lymph node B cells kunye ne-monocytes.Uhlalutyo lwe-ITLC lokubunjwa kwe-GSL ye-B cell neoplasms entsha usebenzisa i-mAb 8281 iqinisekisile ubukho be-lactosylceramide kunye ne-galactosylceramide kwii-neoplasms zezigaba ezahlukeneyo zokwahlula.Ngenxa yobume bayo kwi-terminal βGal carbohydrate iintsalela, i-mAb 8281 inokuba luncedo kuhlalutyo lwesakhiwo kunye nokusebenza kwe-GSL.
