Iimveliso

I-metabolite ye-bacterial intestinal ye-ginseng protopanaxadiol saponin, i-20-O- (β-D-glucopyranosyl) -20 (S) -protopanaxadiol (i-compound K), kuye kwabikwa ukuba yenze i-apoptosis kwiindidi ezahlukeneyo zomhlaza.Nangona kunjalo, iindlela ezichanekileyo ezenziwe yi-compound K kwiiseli ze-hepatocellular carcinoma (HCC) zihlala zingacacanga.Ukuze sihlolisise iindlela ezinokubakho ze-apoptotic, siye saphanda umphumo we-anticancer we-compound K kwi-MHCC97-H.Uvavanyo lwe-MTT lubonise ukuba i-compound K inqanda ukwanda kweeseli ze-MHCC97-H ezinetyhefu ephantsi kwiiseli eziqhelekileyo ze-hepatoma.Ukuqhubela phambili komjikelo weseli kunye nokungcoliswa kweeseli kubonise ukwanda kweqhezu le-apoptotic sub-G1.Unyango lwe-MHCC97-H kunye ne-K ye-compound iphinde ibangele ukunciphisa kwi-membrane ye-mitochondrial (Δψm) kunye nomonakalo we-DNA.Uphononongo olongezelelweyo lubonise ukuba ikhompawundi K ilawula i-Fas, i-FasL, i-Bax / Bcl-2 ratio kunye ne-pro-caspase-9 ye-pro-caspase-9, i-pro-caspase-3 ngendlela exhomekeke kwidosi, kwaye yathintela i-Akt phosphorylation.Ezi ziphumo zibonisa ukuba ikhompawundi i-K inqanda kakhulu ukwanda kweeseli kwaye yenza i-apoptosis kwiiseli ze-MHCC97-H ngokusebenzisa i-Fas- kunye ne-mitochondria-mediated-dependent caspase pathways kwiiseli ze-HCC zabantu.