Iimveliso

I-Bromelain (BR) yi-cysteine ​​​​protease enemiphumo yokuthintela kwi-intestinal secretion kunye nokuvuvukala.Nangona kunjalo, iziphumo zayo kwi-intestinal motility ayikhange ihlolwe.Ngaloo ndlela, siye saphanda umphumo wale khompawundi ephuma kwisityalo kwi-contractility yamathumbu kunye nokuhamba kwiigundane.I-Contractility in vitro yavavanywa ngokuvuselela i-ileum eyodwa yegundane, kwindawo yokuhlambela yamalungu, kunye ne-acetylcholine, i-barium chloride, okanye i-stimulation field field.Ukuhamba kwe-vivo kulinganiswe ngokuvavanya usasazo lwesiphawuli sefluorescent esilawulwa ngomlomo ecaleni kwamathumbu amancinci.I-Transit nayo yavavanywa kwiindawo ze-pathophysiologic ezibangelwa yi-pro-inflammatory ioli ye-croton okanye i-agent ye-diabetogenic streptozotocin.I-Bromelain inqanda ukunyanzeliswa okubangelwa yimichiza ehlukeneyo ye-spasmogenic kwi-ileum ye-mouse enamandla afanayo.Impembelelo ye-antispasmodic yancitshiswa okanye iyachaswa yi-proteolytic enzyme inhibitor, i-gabexate (15 × 10 (-6) i-mol L (-1)), i-protease-activated r eceptor-2 (PAR-2) antagonist, N (1) -3 -methylbutyryl-N (4) -6-aminohexanoyl-piperazine (10(-4) mol L (-1)), i-phospholipase C (PLC) inhibitor, neomycin (3 × 10 (-3) mol L (-1) ) , kunye ne-phosphodiesterase 4 (PDE4) inhibitor, i-rolipram (10 (-6) mol L (-1) ).Kwi-vivo, i-BR ngokukhethekileyo inhibited motility kwi-pathophysiologic states ngendlela ye-PAR-2-antagonist-sensitive.Idatha yethu ibonisa ukuba i-BR inqanda ukuhamba kwamathumbu emathunjini - ngokukhethekileyo kwiimeko ze-pathophysiologic - kunye nesixhobo esinokuthi sibandakanye i-membrane PAR-2 ​​kunye ne-PLC kunye ne-PDE4 njenge-intracellular. iimpawu.I-Bromelain inokuba yinto ekhokelayo ekuphuhlisweni kwamachiza amatsha, ekwazi ukulungelelanisa ukuhamba kwamathumbu ekudumbeni kunye nesifo seswekile.