Iimveliso

I-1.Ukumodareyithwa komsebenzi we-enzymes ehambelana ne-carbohydrate metabolism ibalulekile kwi-potato-induced sweetening (CIS).Inoveli ye-RING finger gene i-SbRFP1 yenziwe i-cloned kwaye ibonakaliso yayo ifunyenwe ukubanda-inducible kwiibhubhu zetapile ze-CIS-resistant genotypes.Ukuguqulwa kwe-SbRFP1 kwiifatata kuqinisekisile indima yayo ekuvimbeleni i-β-amylase kunye nomsebenzi we-invertase, okwathi ngenxa yoko yanciphisa isitashi kunye ne-sucrose degradation kunye nokuqokelela kokunciphisa ishukela kwiibhubhu ezigciniweyo ezibandayo.Ezi ziphumo zibonisa ngokucacileyo ukuba i-SbRFP1 inokusebenza njengomlawuli ongalunganga we-BAM1 kunye ne-StvacINV1 ukunciphisa ukuqokelela kokunciphisa ishukela kwinkqubo ye-potato CIS.

I-2.Injongo yalo msebenzi yayikukufunda impembelelo yokupheka kwe-steam kwi-pectin methylesterase (PME) kunye nemisebenzi engapheliyo ye-α- kunye ne-β-amylase kwii-tissue ezahlukeneyo (i-cortex kunye ne-pith) ye-cv eluhlaza kunye nokushisa.Agria.Kukhethwe amaqondo obushushu amathathu ohlukeneyo okupheka (55, 70 kunye nama-85 °C).Kulingo ngalunye lokupheka, iiprofayili zexesha lobushushu zarekhodwa kwaye iqondo lokupheka lachazwa ngokwemibandela yokupheka.Ukupheka ngomphunga kube negalelo ekwenzeni i-PME isebenze kakhulu kwi-55 °C kunye nokunciphisa umsebenzi wayo kwiqondo lokugqibela lokulungisa (85 °C) , ngeyona mali iphezulu kwi-cortex (0.3745 ± 0.0007 µmol galacturonic acid (GA) g(-1) ubunzima obutsha (FW) min(-1) ) xa kuthelekiswa nepith (0.2617 ± 0.0012 µmol GA g(-1) FW umz(-1) ).Ubukho be-heat-labile kunye nee-isoforms ezizinzile zobushushu ze-PME kwizicubu zetapile eziqwalaselwayo nazo zacingelwa.Ukunyangwa kobushushu ngomphunga kubangele ukwehla okubonakalayo kwimisebenzi engapheliyo ye-α- kunye ne-β-amylase kuzo zombini izihlunu xa kuthelekiswa nepotata ekrwada ukuya kuyo, nangona ngaphandle kokuvalwa ngokupheleleyo.Ii-enzymes zokunciphisa isitatshi nazo zafunyanwa zisasazwa ngokwahlukileyo kwi-tuber ekrwada.Ukupheka komphunga kuchaphazeleke ngeendlela ezahlukeneyo umsebenzi ovavanyiweyo wentsalela ye-enzymatic kwiithishu eziqwalaselweyo zeetapile cv.Agria.Uphando olongezelelweyo luyafuneka ukuqinisekisa iziphumo ezifunyenweyo.